AFP Polyclonal Antibody
规格: | / 50μL / 100μL |
价格: | / ¥1010 / ¥1640 |
货号:E-AB-40293
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
Synonyms | Alpha-fetoprotein,Alpha-1-fetoprotein,Alpha-fetoglobulin,Afp |
Swissprot | P02772 |
Source | Rabbit |
Reactivity | Human,Mouse,Rat |
Immunogen | Recombinant Mouse Afp protein expressed by E.coli |
Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
Recommended dilution | WB,,1:500-1:1000;IHC,,1:200-1:500;IF,,1:100-1:200; |
Concentration | 1.1mg/mL |
Clonality | Polyclonal |
Properties
Cellular localization | Secreted |
Tissue specificity | Plasma.Highest expression level in embryo. |
Isotype | IgG |
Purification | Affinity purification |
Conjugation | Unconjugated |
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
Storage buffer | PBS with 0.05% Proclin300, 50% glycerol, pH7.3. |
Background | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. |
Images
Western blot with Afp Polyclonal antibody at dilution of 1:1000.lane 1:Hep G2 whole cell lysate,lane 2:Mouse placenta,lane 3:Rat placenta,lane 4:Mouse Fetal liver,lane 5:Rat Fetal liver,lane 6: Mouse liver,lane 7:Mouse kidney,lane 8:Rat liver,lane 9:Rat kidney
Immunohistochemistry of paraffin-embedded Mouse liver using AFP Polyclonal Antibody at dilution of 1:50