Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
谷丙转氨酶(ALT/GPT)比色法测试盒
| 规格： | / 48T(18 samples) / 96T(42 samples) |
| 价格： | / ￥200 / ￥320 |
货号：E-BC-K235-M
检测仪器及波长: 酶标仪（500-520 nm）
样本类型: 血清（浆）;动物组织;细胞;细胞上清
检测原理
在37℃、pH7.4条件下，谷丙转氨酶（ALT）催化丙氨酸与α-酮戊二酸之间的氨基转换反应，生成丙酮酸和谷氨酸，反应时间过后，加入苯肼，与丙酮酸生成苯腙，苯腙在碱性条件下呈红棕色。
关键信息
| 指标简称 | ALT,GPT |
| 样本类型 | 血清（浆）;动物组织;细胞;细胞上清 |
| 灵敏度 | 0.75 IU/L |
| 检测范围 | 0.75-72.3 IU/L |
| 精密度 | 批间差：9%批内差：5.3% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择差异较大的2-3个样本稀释成不同浓度进行预实验，结合本试剂盒的检测范围（0.75-72.30 IU/L），选取最佳稀释倍数，进行正式批量实验。不同样本的稀释比例范围如下表（仅供参考）:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
10%大鼠脑匀浆 |
不稀释 |
|
人血浆 |
不稀释 |
10%大鼠心匀浆 |
不稀释 |
|
猪血清 |
不稀释 |
10%大鼠肝匀浆 |
40-60 |
|
大鼠血清 |
不稀释 |
10%大鼠肾匀浆 |
不稀释 |
注：稀释液为生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7.4）。
相关产品
| 货号 | 产品名称 |
|---|---|
| E-BC-K236-M | 谷草转氨酶(AST/GOT)比色法测试盒 |
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