AMPK alpha2 Polyclonal Antibody
Price:
Size:
100μL
50μL
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC;IF
Verified Samples |
Verified Samples in WB:Human Hep G2,Human Hela,Mouse NIH/3T3 Verified Samples in IHC:Human breast Verified Samples in IF:Human MCF7 |
Dilution | WB 1:500-1:1000;IHC 1:100-1:200;IF 1:100-1:400 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Recombinant Rat Ampk2 protein expressed by E.coli |
Abbre | AMPK |
Synonyms | 5'-AMP-activated protein kinase catalytic subunit alpha-2;AAPK2;ACACA kinase;Acetyl-CoA carboxylase kinase;AMPK alpha 2 chain;AMPK subunit alpha-2;AMPK2;AMPKa2;AMPKalpha2;HMGCR kinase;Hydroxymethylglutaryl-CoA reductase kinase;PRKAA;PRKAA2;Protein kinase AMP activated alpha 2 catalytic subunit;Protein kinase AMP activated catalytic subunit alpha 2 |
Swissprot | |
Calculated MW | 62 kDa |
Observed MW | 62 kDa |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH 7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Cardiovascular; Metabolism; Signal Transduction |
Conjugation | Unconjugated |
Background | The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. |
Western blot with Ampk2 Polyclonal antibody at dilution of 1:1000.lane 1:Hep G2 whole cell lysate, lane 2:Hela whole cell lysate, lane 3:NIH/3T3 whole cell lysate
Immunohistochemistry of paraffin-embedded Human breast using AMPK alpha2 Polyclonal Antibody at dilution of 1:100
Immunofluorescence analysis of McF7 cells using AMPK alpha2 Polyclonal Antibody at dilution of 1:100
Immunofluorescence analysis of MCF7 cells using AMPK alpha2 Polyclonal Antibody at dilution of 1:100