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Annexin V-FITC/PI Apoptosis Kit

Annexin V-FITC / PI细胞凋亡检测试剂盒

规格： / 20Assays / 50Assays / 100Assays / 200Assays
价格： / ￥450 / ￥900 / ￥1500 / ￥2400

货号：E-CK-A211

  • 详情
  • 文献(120)

    • 检测原理

    Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸PS有高度亲和力，可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合，将Annexin V标记荧光染料，利用流式细胞仪检测细胞凋亡。 细胞核染料（PI,DAPI,7-AAD）可与双链DNA特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，细胞核染料（DAPI、PI、7-AAD）可进入细胞内对DNA进行染色，与Annexin V搭配使用，可区分处于不同凋亡时期的细胞。

    基本信息

    应用类型 细胞凋亡-Annexin V
    检测方法 荧光
    样本类型 细胞
    检测时间 40min
    检测仪器 流式细胞仪
    荧光 FITC
    Ex/Em (nm) 490/530
    Channel set FITC
    自备试剂 PBS(E-BC-R187), 去离子水
    保存温度 2~8°C, shading light
    运输条件 冰袋
    保质期 12 months

    操作指南

    客户发表文献

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