APC Anti-Mouse CD206/MMR Antibody[C068C2]
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- 应用性: FCM;ICFCM
- 亚型: Rat IgG2a, κ
- 宿主: Rat
Background | CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature. |
Alternate Names | MMR;MR;MRC1;macrophage mannose receptor;mannose receptor ; |
Swissprot | |
Gene ID | |
Clone No | |
Host | Rat |
Reactivity | Mouse |
Application | |
Isotype | Rat IgG2a, κ |
Isotype Control | |
Conjugation | APC |
Conjugation Information | APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). |
Spectrum | |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA. |
Storage | This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. |
Expiration date | 12个月 |
Shipping |
冰袋 |
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IF: 2.833
E-AB-F1135C | FITC Anti-Mouse CD206/MMR Antibody[C068C2] | 100Tests , 50Tests , 100Tests×2 |
E-AB-F1135D | PE Anti-Mouse CD206/MMR Antibody[C068C2] | 100Tests , 50Tests , 100Tests×2 |
E-AB-F1135H | PE/Cyanine7 Anti-Mouse CD206/MMR Antibody[C068C2] | 100Tests , 50Tests , 100Tests×2 |
E-AB-F1135UC | FITC Anti-Mouse CD206/MMR Antibody[C068C2] | 100μg , 25μg |
E-AB-F1135UD | PE Anti-Mouse CD206/MMR Antibody[C068C2] | 100μg , 25μg |
E-AB-F1135UE | APC Anti-Mouse CD206/MMR Antibody[C068C2] | 100μg , 25μg |
E-AB-F1135UH | PE/Cyanine7 Anti-Mouse CD206/MMR Antibody[C068C2] | 100μg , 25μg |
Q1:Is it necessary to take fixation/permeabilization operation while detecting CD206?
CD206 is a transmembrane protein, which can be expressed on both side of the cell membrane. With or without permeabilization are both avaliable for CD206 detection as our anti-mouse CD206 flow antibody can bind both side of CD206. Generally it is recommanded to take fixation/permeabilization operation but these two alternative processes can be decided through the experimental design.
Q2:What are the recommendations for flow cytometry of mouse macrophages to detect M1 and M2 type macrophages?
As a recommandation, F4/80 and CD11b are always used for identyfing macrophages of mouse, and CD86 and CD206 can be used for a further seperation for M1 abd M2 respectively. Specific indicators can be determined according to the literature and experimental requirements.