APC Anti-Mouse F4/80 Antibody[CI:A3-1] E-AB-F0995UE
| 规格： | / 25μg / 50μg / 100μg / 200μg |
| 价格： | / ￥480 / ￥780 / ￥1440 / ￥2640 |
货号：E-AB-F0995UE
货期：咨询
宿主: Rat
应用: FCM
Antigen Information
| Alternate Names | Adhesion G protein-coupled receptor E1,Adgre1,Cell surface glycoprotein F4/80,EGF-like module receptor 1,Adgre1,Emr1, Gpf480,F4/80抗体,F4/80流式抗体,小鼠F4/80,小鼠F4/80抗体,小鼠F4/80流式抗体,F480抗体,F480流式抗体,小鼠F480,小鼠F480抗体,小鼠F480流式抗体 |
| Uniprot ID | Q61549 |
| Background | F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance. |
Product Details
| Form | Liquid |
| Size | 25μg |
| Clone No. | CI:A3-1 |
| Host | Rat |
| Isotype | Rat IgG2b, κ |
| Reactivity | Mouse |
| Application | FCM |
| Isotope Control |
APC Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843E] |
| Storage Buffer | PBS with 0.05% Proclin300, 1% BSA |
| Shipping | Biological ice pack at 4 ℃ |
| Stability & Storage | Keep as concentrated solution. Store at 2~8℃ and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Fluorophore
Conjugation: APC
Recommended Use
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results or refer to the dilution we used in the experiment.
Related Protocols
Troubleshooting
References
1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.