BRCA2 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-40288
宿主: Rabbit
反应性: H,R
应用: WB,IHC,IF
Overview
| Synonyms | BRCA 2, BRCC 2, Breast and ovarian cancer susceptibility gene early onset antibody, BROVCA2, FACD, FAD 1, FANCB, GLM3,BRCA2抗体 |
| Swissprot | P51587 |
| Source | Rabbit |
| Reactivity | Human,Rat |
| Immunogen | Recombinant Human Breast cancer type 2 susceptibility protein |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:1000-1:3000;IHC,,1:100-1:200;IF,,1:100-1:400; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus, Cytoskeleton |
| Tissue specificity | Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% Proclin300, 50% glycerol, pH7.3. |
| Background | Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. |
Images
Western Blot analysis of Jurkat, MCF-7 and A431 cells using BRCA2 Polyclonal Antibody at dilution of 1:2000
Western Blot analysis of Hela cells using BRCA2 Polyclonal Antibody at dilution of 1:2000
Western Blot analysis of Rat testis using BRCA2 Polyclonal Antibody at dilution of 1:2000
Immunohistochemistry of paraffin-embedded Human colon using BRCA2 Polyclonal Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded Human breast using BRCA2 Polyclonal Antibody at dilution of 1:100
