CDK4 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB
| Verified Samples |
Verified Samples in WB:Hela,293T |
| Dilution | WB 1:500-1:2000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human CDK4 |
| Abbre | CDK4 |
| Synonyms | Cdk 4;cdk4;CDK4 protein;CDK4;Cell division kinase 4;Cell division protein kinase 4;CMM 3;CMM3;Crk3;Cyclin dependent kinase 4;Cyclin-dependent kinase 4;Melanoma cutaneous malignant 3;MGC14458;p34 cdk4;PSK J3;PSK-J3抗体 |
| Swissprot | |
| Calculated MW | 34kDa |
| Cellular Localization | Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. |
| Concentration | 0.4 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Cell Biology; Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Background | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. |
Western Blot analysis of Hela and 293T cell using CDK4 Polyclonal Antibody at dilution of 1:450
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Immune regulation mechanism of Astragaloside IV on RAW264.7 cells through activating the NF-κB/MAPK signaling pathway
IF: 2.956Journal：INTERNATIONAL IMMUNOPHARMACOLOGY
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
