DDX1 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human; Mouse; Rat
- 应用性： WB
| Verified Samples |
Verified Samples in WB:Mouse brain,Rat brain |
| Dilution | WB 1:500-1:2000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Fusion protein of human DDX1 |
| Abbre | DDX1 |
| Synonyms | ATP dependent helicase DDX1;ATP-dependent RNA helicase DDX1;DBP RB ;DBP-RB;DDX1;DDX1;DEAD (Asp Glu Ala Asp) box polypeptide 1;DEAD box 1;DEAD box polypeptide 1;DEAD box protein 1;DEAD box protein retinoblastoma;DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1抗体 |
| Swissprot | |
| Calculated MW | 82 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasmic granule. Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev. |
| Concentration | 0.72 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Background | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. |
Western blot analysis of Mouse brain tissue and Rat brain tissue lysates using DDX1 Polyclonal Antibody at dilution of 1:300
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
