ERK 1/2 Polyclonal Antibody
Price:
Size:
100μL
50μL
- 宿主： Rabbit
- 反应性： Mouse
- 应用性： WB
| Verified Samples |
Verified Samples in WB:NIH/3T3 |
| Dilution | WB 1:500-1:1000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Rat ERK 1/2 protein expressed by E.coli |
| Abbre | ERK 1/2 |
| Synonyms | Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;ERT2;Extracellular signal-regulated kinase 1 (ERK-1);Insulin-stimulated MAP2 kinase;MAP kinase isoform p44 (p44-MAPK);MNK1;Microtubule-associated protein 2 kinase;p44-ERK1;Mapk3;Erk1;Prkm3 |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW | 43 kDa |
| Concentration | 1mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide,50% glycerol pH 7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Background | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases,also known as extracellular signal-regulated kinases (ERKs),act in a signaling cascade that regulates various cellular processes such as proliferation,differentiation,and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases,resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described,This gene encodes a member of the MAP kinase family. MAP kinases,also known as extracellular signal-regulated kinases (ERKs),act as an integration point for multiple biochemical signals,and are involved in a wide variety of cellular processes such as proliferation,differentiation,transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation,this kinase translocates to the nucleus of the stimulated cells,where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein,but differing in the UTRs,have been reported for this gene. |
Western blot with ERK 1/2 Polyclonal Antibody at dilution of 1:1000.lane 1:NIH/3T3 whole cell lysate
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
