Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)
葡萄糖(Glu)比色法测试盒(GOD-POD法)
规格: | / 48T(32 samples) / 96T(80 samples) |
价格: | / ¥200 / ¥320 |
货号:E-BC-K234-M
检测仪器及波长: 酶标仪(500-510 nm)
样本类型: 全血;血清;血浆
检测原理
葡萄糖氧化酶(GOD,EC 1.1.3.4)能催化葡萄糖氧化成葡萄糖酸,产生过氧化氢,在色原性氧受体的存在下,过氧化物酶催化过氧化氢,氧化色素源,生成有色物质。
关键信息
指标简称 | Glu |
样本类型 | 全血;血清;血浆 |
灵敏度 | 0.04 mmol/L |
检测范围 | 0.04-30 mmol/L |
精密度 | 批间差:2.3%批内差:1.9% |
检测仪器及波长 | 酶标仪 |
保存条件 | 2-8℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择2-3例预期差异大的样本稀释成不同浓度进行预实验,结合本试剂盒的线性范围(0.04-30 mmol/L),选取最佳稀释倍数,进行正式批量实验。不同样本的稀释比例范围如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
人血清 |
不稀释 |
大鼠血清 |
不稀释 |
小鼠血清 |
不稀释 |
人血浆 |
不稀释 |
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