Glutathione Peroxidase (GSH-Px) Activity Assay Kit
谷胱甘肽过氧化物酶(GSH-Px)比色法测试盒
规格: | / 48T(16 samples) / 96T(40 samples) |
价格: | / ¥480 / ¥860 |
货号:E-BC-K096-M
检测仪器及波长: 酶标仪(400-420 nm,最佳检测波长412 nm)
样本类型: 血清;血浆;培养细胞;培养液及动植物组织
检测原理
GSH-Px可以促进过氧化氢(H2O2)与还原型谷胱甘肽(GSH)反应生成H2O及氧化型谷胱甘肽(GSSG),反应式见下图。通过测定底物GSH在单位时间内被氧化的速度来表示酶的活力,反应剩余的GSH与二硫代双二硝基苯甲酸(DTNB)作用生成的黄色硫代硝基苯甲酸阴离子(TNB),测定该阴离子的浓度来计算出GSH的减少量。由于GSH在没有酶的条件下也能进行氧化(非酶反应),所以最后计算此酶活力时,必须扣除非酶反应引起的GSH减少的那部分。
关键信息
指标简称 | GSH-PX,GPX |
样本类型 | 血清;血浆;培养细胞;培养液及动植物组织 |
灵敏度 | 34.34 U |
检测范围 | 34.34-1036.64 U |
精密度 | 批间差:8.7%批内差:2.4% |
检测仪器及波长 | 酶标仪 |
保存条件 | 2-8℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择2-3个预期差异较大的样本稀释成不同浓度,选取抑制率在25%-45%的样本的稀释倍数,进行正式批量实验,不同样本稀释比例范围如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
人血清 |
不稀释 |
10%大鼠肝脏匀浆 |
30-60 |
小鼠血清 |
3-5 |
HepG2细胞匀浆(5mgprot/mL) |
不稀释 |
10%小鼠脑匀浆 |
不稀释 |
10%绿萝叶组织匀浆 |
不稀释 |
大鼠血清 |
5-8 |
10%小白菜叶组织匀浆 |
3-5 |
注:稀释液生理盐水(0.9% NaCl)或PBS(0.01 M,pH 7.4)。
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