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Glutathione Peroxidase (GSH-Px) Activity Assay Kit

谷胱甘肽过氧化物酶(GSH-Px)比色法测试盒

规格: / 48T(16 samples) / 96T(40 samples)
价格: / ¥480 / ¥860

货号:E-BC-K096-M

检测仪器及波长: 酶标仪(400-420 nm,最佳检测波长412 nm)

样本类型: 血清;血浆;培养细胞;培养液及动植物组织

  • 详情
  • 文献(54)
  • 检测原理

    GSH-Px可以促进过氧化氢(H2O2)与还原型谷胱甘肽(GSH)反应生成H2O及氧化型谷胱甘肽(GSSG),反应式见下图。通过测定底物GSH在单位时间内被氧化的速度来表示酶的活力,反应剩余的GSH与二硫代双二硝基苯甲酸(DTNB)作用生成的黄色硫代硝基苯甲酸阴离子(TNB),测定该阴离子的浓度来计算出GSH的减少量。由于GSH在没有酶的条件下也能进行氧化(非酶反应),所以最后计算此酶活力时,必须扣除非酶反应引起的GSH减少的那部分。

    关键信息

    指标简称 GSH-PX,GPX
    样本类型 血清;血浆;培养细胞;培养液及动植物组织
    灵敏度 34.34 U
    检测范围 34.34-1036.64 U
    精密度 批间差:8.7%批内差:2.4%
    检测仪器及波长 酶标仪
    保存条件 2-8℃
    有效期 6个月

    样本稀释倍数

    在正式检测前,需选择2-3个预期差异较大的样本稀释成不同浓度,选取抑制率在25%-45%的样本的稀释倍数,进行正式批量实验,不同样本稀释比例范围如下表(仅供参考):

    样本

    稀释倍数

    样本

    稀释倍数

    人血清

    不稀释

    10%大鼠肝脏匀浆

    30-60

    小鼠血清

    3-5

    HepG2细胞匀浆(5mgprot/mL

    不稀释

    10%小鼠脑匀浆

    不稀释

    10%绿萝叶组织匀浆

    不稀释

    大鼠血清

    5-8

    10%小白菜叶组织匀浆

    3-5

    :稀释液生理盐水(0.9% NaCl)或PBS(0.01 M,pH 7.4)。


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