Glutathione Reductase (GR) Activity Assay Kit
谷胱甘肽还原酶(GR)比色法测试盒
| 规格： | / 50 assays(48 samples) / 100 assays(96 samples) |
| 价格： | / ￥380 / ￥680 |
货号：E-BC-K099-S
检测仪器及波长: 紫外-可见光分光光度计（340 nm）
样本类型: 血清;血浆;组织;细胞
检测原理
由还原型辅酶（NADPH）供氢，催化氧化型谷胱甘肽（GSSG），还原成还原型谷胱甘肽（GSH），在340 nm处，通过检测NADPH的改变，可计算出GR的活力。
关键信息
| 指标简称 | GR |
| 样本类型 | 血清;血浆;组织;细胞 |
| 灵敏度 | 6.2 U/L |
| 检测范围 | 6.2-320 U/L |
| 精密度 | 批间差：2.5%批内差：2.1% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | Reagent 1 2-8℃，其他-20℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，结合本试剂盒的检测范围（6.2-320 U/L），选取最佳稀释倍数，进行正式批量实验。不同样本的稀释比例范围如下表（仅供参考）:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
大鼠血清 |
不稀释 |
|
人血浆 |
不稀释 |
大鼠血浆 |
不稀释 |
|
小鼠血清 |
不稀释 |
10%小鼠肝匀浆 |
1-3 |
|
小鼠血浆 |
不稀释 |
10%绿萝匀浆 |
不稀释 |
注：稀释液为双蒸水或生理盐水（0.9% NaCl）。
客户发表文献
- 文献: In vitro and in vivo anticancer effects of syringic acid on colorectal cancer: Possible mechanistic view
样品类型: Cell sample - 文献: Prenatal exposure to environmental pro-oxidants induces mitochondria-mediated epigenetic changes: a cross-sectional pilot study
样品类型: plasma - 文献: Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats
样品类型: serum - 文献: Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats
样品类型: Tissue homogenate - 文献: Metal accumulation in muscle and oxidative stress response in the liver of juvenile Oreochromis niloticus from contaminated sediment under a simulation of increasing temperature
样品类型: liver - 文献: Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis
样品类型: Tissue homogenate - 文献: Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades
样品类型: cell - 文献: Neuroprotective effect of bromelain in 6-hydroxydopamine induced in vitro model of Parkinson’s disease
样品类型: SH-SY5Y cell - 文献: Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains
样品类型: brain
