Histone H3 Polyclonal Antibody
Price:
Size:
200μL
100μL
50μL
20μL
- 宿主： Rabbit
- 反应性： Human; Mouse; Rat
- 应用性： WB
| Verified Samples |
Verified Samples in WB:Mouse Spleen,HT-29,NIH/3T3,C6 |
| Dilution | WB 1:5000-10000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide derived from N-terminus of human Histone H3 protein conjugated to keyhole limpet haemocyanin. |
| Abbre | Histone H3 |
| Synonyms | H3 histone family; member A; H3/A; H31; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1; H3a; Histone cluster 1; H3a; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l抗体 |
| Swissprot | |
| Calculated MW | 15kDa |
| Observed MW | 15kDa |
| Cellular Localization | Chromosome, Nucleosome core, Nucleus |
| Concentration | 2.5mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol,pH 7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Background | Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. This antibody is not cross-reactive with other histones. |
Western blot of Mouse spleen lysates with anti-Histone H3 pAb at dilution of 1:1000.
Western Blotting with anti-Histone H3 polyclonal antibody at dilution of 1:5000.
Lane 1:HT-29 cell lysates, Lane 2: NIH/3T3 cell lysates, Lane 3: C6
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
