HSP27 Monoclonal Antibody
Price:
Size:
120μL
60μL
20μL
- 宿主： Mouse
- 反应性： Human
- 应用性： WB;IHC-p
| Verified Samples |
Verified Samples in WB:Hela Verified Samples in IHC:Human breast carcinoma |
| Dilution | WB 1:1000-1:3000;IHC 1:50-1:200 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Recombinant Protein |
| Abbre | HSP27 |
| Synonyms | Heat shock 27kDa protein;28 kDa heat shock protein;CMT2F;DKFZp586P1322;epididymis secretory protein Li 102;Estrogen regulated 24 kDa protein;Estrogen-regulated 24 kDa protein;Heat shock 25kDa protein 1;Heat shock 27 kDa protein;Heat shock 27kD protein 1;Heat shock 27kDa protein 1;Heat shock 28kDa protein 1;Heat Shock Protein 27;Heat shock protein beta 1;Heat shock protein beta-1;heat shock protein family B (small) member 1;HEL-S-102;HMN2B;HS.76067;Hsp 25;HSP 27;Hsp 28;Hsp B1;Hsp25;HSP27;Hsp28;HspB1;HSPB1;SRP27;Stress responsive protein 27;Stress-responsive protein 27抗体 |
| Swissprot | |
| Observed MW | 27kDa |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm>cytoskeleton>spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. |
| Tissue Specificity | Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification Method | Protein A purification |
| Research Areas | Cancer; Cardiovascular; Signal Transduction |
| Clone No. | Clone:3E5 |
| Conjugation | Unconjugated |
| Background | Involved in stress resistance and actin organization. |
Western Blot analysis of Hela cells using HSP27 Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using HSP27 Monoclonal Antibody at dilution of 1:200.
| E-AB-1001 | Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1024 | Goat Anti-Mouse IgG(H+L)(Biotin conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1196 | HRP-Goat Anti-Mouse IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
