Human TIMP-2(Tissue Inhibitors of Metalloproteinase 2) ELISA Kit
中文名称:人基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫吸附测定试剂盒
别称:
TIMP2、CSC-21K
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人TIMP-2抗体包被于酶标板上,实验时样品(或标准品)中的人TIMP-2会与包被抗体结合。后依次加入生物素化的抗人TIMP-2抗体和辣根过氧化物酶标记的亲和素,抗人TIMP-2抗体与结合在包被抗体上的人TIMP-2结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TIMP-2浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TIMP-2的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人TIMP-2,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis
IF: 7.419Journal:BIOMEDICINE & PHARMACOTHERAPY -
Three kinds of corneal host cells contribute differently to corneal neovascularization
IF: 6.68 -
The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases
IF: 6.6Journal:Cells -
TIMP-2 regulates 5-Fu resistance via the ERK/MAPK signaling pathway in colorectal cancer
IF: 5.682 -
Urinary NGAL, IGFBP-7, and TIMP-2: novel biomarkers to predict contrast medium-induced acute kidney injury in children
IF: 3.222 -
MT1‐MMP evaluation in neointimal hyperplasia in the late follow‐up after prosthesis implantation
IF: 1.494Journal:INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY