Hydrogen Peroxide (H2O2) Colorimetric Assay Kit
过氧化氢(H2O2)比色法测试盒
| 规格： | / 48T(32 samples) / 96T(80 samples) |
| 价格： | / ￥220 / ￥350 |
货号：E-BC-K102-M
检测仪器及波长: 酶标仪（402-407 nm，最适检测波长405nm）
样本类型: 血清;血浆;尿液;动植物组织及细胞
检测原理
H2O2与钼酸铵反应生成稳定的黄色络合物且在405 nm处有最大吸收，黄色络合物的颜色深浅与H2O2的浓度在一定范围内具有线性关系。故可以通过比色计算出H2O2的含量。
关键信息
| 指标简称 | H2O2 |
| 样本类型 | 血清;血浆;尿液;动植物组织及细胞 |
| 灵敏度 | 0.41 mmol/L |
| 检测范围 | 0.41-125 mmol/L |
| 精密度 | 批间差：3.6%批内差：3.2% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，不同样本稀释如下表（仅供参考）：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
细胞匀浆 |
不稀释 |
|
大鼠血清 |
不稀释 |
10%植物组织 |
不稀释 |
|
小鼠血清 |
不稀释 |
10%大鼠心组织 |
不稀释 |
|
小鼠血浆 |
不稀释 |
10%大鼠脑组织 |
不稀释 |
|
猪血清 |
不稀释 |
10%大鼠肾组织 |
不稀释 |
|
人尿液 |
不稀释 |
10%大鼠肝组织 |
不稀释 |
注：稀释液为生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7. 4 ）。
相关产品
| 货号 | 产品名称 |
|---|---|
| E-BC-F001 | 过氧化氢(H2O2)荧光法测试盒 |
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