L-Lactic Acid (LA) Colorimetric Assay Kit
L-乳酸(LA)比色法测试盒
| 规格： | / 48T(32 samples) / 96T(80 samples) |
| 价格： | / ￥620 / ￥1100 |
货号：E-BC-K044-M
检测仪器及波长: 酶标仪（520-540 nm，最适检测波长530 nm）
样本类型: 血清（浆）;细胞上清;组织;细胞
检测原理
以氧化型辅酶Ⅰ（NAD+）为氢受体，乳酸脱氢酶（LDH）催化乳酸脱氢产生丙酮酸，使NAD+转化为还原型辅酶Ⅰ（NADH）。其中N-甲基吩嗪甲基硫酸盐（PMS）递氢使氯化硝基四氮唑蓝（NBT）还原为紫色呈色物，呈色物的吸光度在530 nm时与乳酸含量呈线性关系。
关键信息
| 样本类型 | 血清（浆）;细胞上清;组织;细胞 |
| 灵敏度 | 0.10 mmol/L |
| 检测范围 | 0.12-7.0 mmol/L |
| 精密度 | 批间差：3.5%批内差：1.4% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择样本之间差异较大的2-3例稀释成不同浓度进行预实验，不同样本稀释比例如下表（仅供参考）：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
2-5 |
HepG2细胞上清 |
不稀释 |
|
10%大鼠肾脏组织 |
1-3 |
HepG2细胞 |
不稀释 |
|
10%大鼠脑组织 |
不稀释 |
|
|
注：稀释液为生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7.4）
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