Lactate Dehydrogenase (LDH) Activity Assay Kit
乳酸脱氢酶(LDH)比色法测试盒
| 规格： | / 96T(40 samples) |
| 价格： | / ￥500 |
货号：E-BC-K046-M
检测仪器及波长: 酶标仪（440-460 nm，最佳检测波长450 nm）
样本类型: 血清;血浆;胸水;组织;细胞
检测原理
以辅酶I为递氢体，LDH催化乳酸产生丙酮酸，丙酮酸与2,4-二硝基苯肼作用生成丙酮酸二硝基苯腙，后者在碱性溶液中呈棕红色，颜色的深浅与丙酮酸的浓度呈正比，通过测定OD值，可计算LDH的活力。
关键信息
| 指标简称 | LDH |
| 样本类型 | 血清;血浆;胸水;组织;细胞 |
| 灵敏度 | 6 U/L |
| 检测范围 | 6-500 U/L |
| 精密度 | 批间差：2.4%批内差：1.8% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3例预期差异大的样本稀释成不同浓度进行预实验，根据预实验的结果，结合本试剂盒的检测范围（6-500 U/L），请参考下表稀释：
|
LDH活力（U/L） |
样本与稀释液的体积比 |
稀释倍数 |
|
<500 |
不稀释 |
1 |
|
500-5000 |
1:9 |
10 |
注：稀释液为双蒸水或生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7.4）。
常见样本的稀释倍数表：
|
样本 |
稀释倍数 |
|
人血清 |
10倍稀释 |
|
人血浆 |
15-30倍稀释 |
|
猪血清 |
10-20倍稀释 |
|
人胸水 |
5-8倍稀释 |
|
10%小鼠肾组织匀浆 |
500-800倍稀释 |
|
10%小鼠肺组织匀浆 |
500倍稀释 |
|
10%小鼠肝组织匀浆 |
500-800倍稀释 |
|
HepG2细胞匀浆 |
100-300倍稀释 |
|
稀释液：PBS（0.01 M，pH 7.4） |
|
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