Lipid Peroxide (LPO) Colorimetric Assay Kit
脂质过氧化物(LPO)比色法测试盒
规格: | / 48T(32 samples) / 96T(80 samples) |
价格: | / ¥1300 / ¥2000 |
货号:E-BC-K176-M
检测仪器及波长: 酶标仪(580-590 nm)
样本类型: 血清(浆);尿液;组织样本
检测原理
在酸性且45℃条件下,脂质过氧化物与显色剂结合,产生稳定的显色基团,在586 nm处有最大吸收峰,在一定范围内吸光度值与浓度成正比。
关键信息
指标简称 | LPO |
样本类型 | 血清(浆);尿液;组织样本 |
灵敏度 | 0.70 μmol/L |
检测范围 | 0.70-80 μmol/L |
精密度 | 批间差:3.5%批内差:3.1% |
检测仪器及波长 | 酶标仪 |
特殊仪器 | 恒温水浴锅 |
保存条件 | 2-8℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择2-3例预期差异大的样本稀释成不同浓度进行预实验,根据预实验的结果,结合本试剂盒的线性范围(0.70-80 μmol/L),请参考下表稀释:
LPO浓度(μmol/L) |
样本与双蒸水的体积比 |
稀释倍数 |
<80 |
不稀释 |
1 |
80-800 |
1:9 |
10 |
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