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Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

丙二醛(MDA)比色法测试盒(TBA法)

规格: / 48T(32 samples) / 96T(80 samples)
价格: / ¥350 / ¥600

货号:E-BC-K025-M

检测仪器及波长: 酶标仪(530-540 nm,最佳532nm)

样本类型: 血清(浆);动物组织

  • 详情
  • 文献(84)
  • 检测原理

    过氧化脂质降解产物中的丙二醛(MDA)在高温及酸性环境下可与硫代巴比妥酸(TBA)反应产生红棕色产物3,5,5’-三甲基恶唑2,4-二酮(三甲川),该物质在532nm处有最大吸收峰。

    关键信息

    指标简称 MDA
    样本类型 血清(浆);动物组织
    灵敏度 1.13 μmol/L
    检测范围 2.92-40 μmol/L
    精密度 批间差:7.2%批内差:4.1%
    检测仪器及波长 酶标仪
    特殊仪器 100℃恒温水浴锅
    自备试剂 冰乙酸、无水乙醇
    保存条件 2-8℃
    有效期 6个月

    样本稀释倍数

    在正式检测前,需选择2-3个预期差异大的样本稀释成不同浓度进行预实验,根据预实验的结果,结合本试剂盒的线性范围(2.92-40 μmol/L),请参考下表稀释:

    样本

    稀释倍数

    样本

    稀释倍数

    人血清

    不稀释

    10%大鼠心匀浆

    不稀释

    人血浆

    不稀释

    10%大鼠肝匀浆

    不稀释

    大鼠血清

    不稀释

    10%大鼠脾匀浆

    不稀释

    大鼠血浆

    不稀释

    10%大鼠肺匀浆

    不稀释

    小鼠血清

    不稀释

    10%大鼠肾匀浆

    不稀释

    小鼠血浆

    不稀释

    10%大鼠脑匀浆

    不稀释

    注:稀释液为生理盐水(0.9% NaCl)或PBS(0.01 M,pH 7.4)。


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