MDA(Malondialdehyde) ELISA Kit

产品货号:E-EL-0060c

规格: / 96T / 48T
价格: / ¥2400 / ¥1650

货号:E-EL-0060c

货期:3个工作日+顺丰周期

反应性: 通用

检测范围: 31.25~2000 ng/mL

灵敏度: 18.75 ng/mL

  • 详情
  • 文献(15)
  • 用途

    该试剂盒用于体外定量检测血清、血浆或其他相关生物液体中天然及部分重组MDA浓度。

    检测原理

    本试剂盒采用竞争ELISA法。用MDA抗原包被于酶标板上,实验时样品或标准品中的MDA与包被的MDA竞争生物素标记的抗MDA单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,MDA浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中MDA的浓度。

    反应类型 竞争法
    规格 96T
    反应时间 2.0h
    反应性 通用
    检测方法 Colormetric
    检测范围 31.25-2000 ng/mL
    灵敏度 18.75 ng/mL
    样本体积 50μL
    样本类型 血清、血浆或其他相关生物液体

    特异性

    可检测样本中的MDA,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (ng/mL) O.D Average
    2000 0.352
    0.398
    0.375
    1000 0.498
    0.508
    0.503
    500 0.741
    0.709
    0.725
    250 1.058
    1.068
    1.063
    125 1.507
    1.481
    1.494
    62.5 1.942
    1.92
    1.931
    31.25 2.284
    2.284
    2.284
    0 2.804
    2.818
    2.811

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (ng/mL)
    109.60 308.70 848.80 111.50 321.00 892.50
    Standard
    deviation
    6.10 17.30 37.30 7.00 17.00 46.40
    C V (%) 5.57 5.60 4.39 6.28 5.30 5.20

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 92-103 97
    EDTA plasma (n=5) 94-107 99
    Cell culture media (n=5) 93-110 100

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 84-99 89-101
    Average (%) 99 91 95
    1:4 Range (%) 82-95 88-103 100-113
    Average (%) 89 95 105
    1:8 Range (%) 86-97 87-102 99-111
    Average (%) 92 94 105
    1:16 Range (%) 85-96 87-99 94-110
    Average (%) 91 92 100

    试剂盒组成及保存

    未拆封的试剂盒可在4℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 4℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 4℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 4℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1.酶标仪(450nm波长滤光片)
    • 2.高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3.37℃恒温箱,双蒸水或去离子水
    • 4.吸水纸

    操作步骤

    1. 对应板孔中加入50μL标准品工作液或样本后,立即每孔加入50μL生物素化抗体工作液,37℃孵育90分钟
    2. 弃掉板内液体,洗板3次
    3. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次
    4. 每孔加入90μL底物溶液,37℃孵育15分钟
    5. 每孔加入50μL终止液
    6. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. 文献: SONG Quan Quan, NIU Jing Ping, ZHANG Shu Yu, LIANG Ting Ting, ZHOU Ji, FENG Shan Shan. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice. Biomedical and Environmental Sciences, 2018, 31(10): 757-768.
      样品类型: Plasma,Tissue homogenate
    2. 文献: Guo X, Fan Y, Cui J, et al. NOX4 expression and distal arteriolar remodeling correlate with pulmonary hypertension in COPD[J]. BMC pulmonary medicine, 2018, 18(1): 111.
      样品类型: Serum
    3. 文献: Oladosu O W, Biliaminu S A, Abdulazeez I M, et al. Assessment of seminal biomarker of lipid peroxidation among male partners of infertile couples at the university of ilorin teaching hospital, Nigeria[J]. Nigerian Postgraduate Medical Journal, 2018, 25(2): 94.
      样品类型: Sperm
    4. 文献: Bahadr A, Ceyhan A, Gergin , et al. Protective Effects of Curcumin and Beta-carotene on Cisplatin-induced Cardiotoxicity: An Experimental Rat Model[J]. Anatolian Journal of Cardiology, 2018, 19(3): 213.
    5. 文献: Franceschelli S, Gatta D M P, Pesce M, et al. Modulation of the oxidative plasmatic state in gastroesophageal reflux disease with the addition of rich water molecular hydrogen: A new biological vision[J]. Journal of Cellular and Molecular Medicine, 2018.
    6. 文献: Kim J, Choung S Y. Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups[J]. Molecular Vision, 2017, 23: 638-648.
      样品类型: Tissue homogenate
    7. 文献: Bai D, Jin G, Yin S, et al. Antioxidative And Anti-Apoptotic Roles Of Silibinin In Reversing Learning And Memory Deficits In APP/PS1 Mice[J]. Neurochemical Research, 2017: 1-7.
      样品类型: Tissue homogenate
    8. 文献: Kzlay Z, Kahraman etin N, Aksel M, et al. Ozone Partially Decreases Axonal And Myelin Damage In An Experimental Sciatic Nerve Injury Model[J]. Journal of Investigative Surgery, 2017.
      样品类型: Serum
    9. 文献: Jeong H, Liu Y, Kim H S. Dried Plum And Chokeberry Ameliorate D-Galactose-Induced Aging In Mice By Regulation Of Pl3K/Akt-Mediated Nrf2 And Nf-kB Pathways[J]. Experimental Gerontology, 2017, 95: 16-25.
      样品类型: Serum
    10. 文献: Tan C, Lu S, Wang Y, et al. Long-Term Exposure To High Air Pollution Induces Cumulative DNA Damages In Traffic Policemen[J]. Science of The Total Environment, 2017, 593: 330-336.
      样品类型: Serum
    11. 文献: Su H M, Feng L N, Zheng X D, et al. Myricetin Protects Against Diet-Induced Obesity And Ameliorates Oxidative Stress In C57BL/6 Mice[J]. Journal of Zhejiang University-Science B, 2016, 17(6): 437-446.
      样品类型: Serum
    12. 文献: Cheng Q, Sun G J, Liu S B, et al. A Novel Translocator Protein 18 kDa Ligand, ZBD-2, Exerts Neuroprotective Effects Against Acute Spinal Cord Injury[J]. Clinical and Experimental Pharmacology and Physiology, 2016, 43(10): 930-938.
      样品类型: Serum
    13. 文献: Luo Y, Fu C, Wang Z, et al. Asiaticoside Attenuates The Effects Of Spinal Cord Injury Through Antioxidant And AntiInflammatory Effects, And Inhibition Of The p38MAPK Mechanism[J]. Molecular Medicine Reports, 2015, 12: 8294-8300.
      样品类型: Serum
    14. 文献: Zhao J, Xu S Z, Song F, et al. 2, 3, 5, 4' -Tetrahydroxystilbene-2-O-β-D-Glucoside Protects Human Umbilical Vein Endothelial Cells Against Lysophosphatidylcholine-Induced Apoptosis By Upregulating Superoxide Dismutase And Glutathione Peroxidase[J]. Iubmb Life, 2014, 66(10): 711-722.
      样品类型: Cell lysate
    15. 文献: Peng Zeng, Yan Shi, Xiao-Ming Wang, Li Lin, Yan-Jun Du, Na Tang, Qun Wang, Ying-Yan Fang, Jian-Zhi Wang, Xin-Wen Zhou, Youming Lu, Qing Tian; Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer’s Disease-Like Features in Rats, International Journal of Neuropsychopharmacology, pyy090.
      样品类型: Serum, Tissue homogenate
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