Mouse ALB(Albumin) ELISA Kit
中文名称:小鼠白蛋白(ALB)酶联免疫吸附测定试剂盒
别称:
Serum albumin、Alb、Alb1、Alb-1
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 62.5-4000 ng/mL
- 灵敏度: 37.5 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠ALB抗体包被于酶标板上,实验时样品(或标准品)中的小鼠ALB会与包被抗体结合。后依次加入生物素化的抗小鼠ALB抗体和辣根过氧化物酶标记的亲和素,抗小鼠ALB抗体与结合在包被抗体上的小鼠ALB结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,ALB浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中ALB的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 62.5-4000 ng/mL |
灵敏度 | 37.5 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠ALB,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
GPR43 deficiency protects against podocyte insulin resistance in diabetic nephropathy through the restoration of AMPKα activity
IF: 11.556Journal:Theranostics -
Supplement of microbiota-accessible carbohydrates prevents neuroinflammation and cognitive decline by improving the gut microbiota-brain axis in diet-induced obese mice
IF: 5.793Journal:Journal of Neuroinflammation -
Oestradiol promotes the intrahepatic bile duct development of C57BL/6CrSlc mice during embryonic period via Notch signalling pathway
IF: 5.31 -
α2A-adrenoceptor deficiency attenuates lipopolysaccharide-induced lung injury by increasing norepinephrine levels and inhibiting alveolar macrophage activation in acute respiratory distress syndrome
IF: 5.223Journal:CLINICAL SCIENCE -
MDM2 Contributes to High Glucose-Induced Glomerular Mesangial Cell Proliferation and Extracellular Matrix Accumulation via Notch1
IF: 4.38Journal:Scientific Reports -
Catalpol Ameliorates Podocyte Injury by Stabilizing Cytoskeleton and Enhancing Autophagy in Diabetic Nephropathy
IF: 3.845Journal:Frontiers in Pharmacology -
Inhibition of LPS-induced Nox2 activation by VAS2870 protects alveolar epithelial cells through eliminating ROS and restoring tight junctions
IF: 2.985Journal:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONSPMID:32019675