Mouse IL-6(Interleukin 6) ELISA Kit

产品货号:E-EL-M0044c

规格: / 96T / 48T
价格: / ¥2400 / ¥1650

货号:E-EL-M0044c

货期:3个工作日+顺丰周期

反应性: 小鼠

检测范围: 31.25~2000 pg/mL

灵敏度: 18.75 pg/mL

  • 详情
  • 文献(28)
  • 用途

    该试剂盒用于体外定量检测小鼠Serum, plasma and other biological fluids中天然及部分重组IL-6浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上,实验时样品或标准品中的小鼠IL-6会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素。抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-6浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-6的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 小鼠
    检测方法 Colormetric
    检测范围 31.25—2000 pg/mL
    灵敏度 18.75 pg/mL
    样本体积 100μL
    样本类型 Serum, plasma and other biological fluids

    特异性

    可检测样本中的小鼠IL-6,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    2000 2.471
    2.503
    2.487 2.427
    1000 1.682
    1.72
    1.701 1.641
    500 0.945
    0.915
    0.93 0.87
    250 0.421
    0.441
    0.431 0.371
    125 0.267
    0.243
    0.255 0.195
    62.5 0.17
    0.154
    0.162 0.102
    31.25 0.109
    0.115
    0.112 0.052
    0 0.05
    0.07
    0.06 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    99.40 174.50 952.80 105.30 162.70 1040.70
    Standard
    deviation
    6.40 7.70 50.50 6.50 8.10 38.50
    C V (%) 6.44 4.41 5.30 6.17 4.98 3.70

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 86-100 93
    EDTA plasma (n=5) 88-101 94
    Cell culture media (n=5) 89-104 95

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 91-106 91-103 98-111
    Average (%) 98 98 104
    1:4 Range (%) 94-109 88-103 98-110
    Average (%) 100 94 104
    1:8 Range (%) 94-108 87-98 95-106
    Average (%) 101 93 100
    1:16 Range (%) 97-113 84-97 97-109
    Average (%) 105 91 103

    试剂盒组成及保存

    未拆封的试剂盒可在4℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 4℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 4℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 4℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1.对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟
    2.弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟
    3.弃掉板内液体,洗板3次
    4.每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次
    5.每孔加入90μL底物溶液,37℃孵育15分钟
    6.每孔加入50μL终止液
    7.立即在450nm波长下读数,处理数据

    客户发表文献

    1. 文献: Tan N, Tong X, Liang R, et al. Ameliorative Effects of Suhuang Antitussive Capsule on LPS-Induced Sputum Obstruction in Mice through Promoting HGF Secretion via an Intestinal Endocrine-Dependent Pathway[J]. Frontiers in Pharmacology, 2019, 10: 1422.
      样品类型: Serum,Tissue homogenate
    2. 文献: Li Z , Hu Y H , Fu Q R , et al. NIR/ROS-Responsive Black Phosphorus QD Vesicles as Immunoadjuvant Carrier for Specific Cancer Photodynamic Immunotherapy[J]. Advanced Functional Materials, 2019.
      样品类型: Serum
    3. 文献: Zhang Y L , Zheng Y , Shi W , et al. Design, Synthesis and Investigation of the Potential Anti-inflammatory Activity of 7-O-Amide Hesperetin Derivatives[J]. Molecules, 2019, 24, 3363.
      样品类型: Cell culture supernatant
    4. 文献: Liu Q , Cai J Q , Zheng Y D , et al. NanoRNP Overcomes Tumor Heterogeneity in Cancer Treatment[J]. NANO LETTERS, 2019
      样品类型: Serum
    5. 文献: SONG Quan Quan, NIU Jing Ping, ZHANG Shu Yu, LIANG Ting Ting, ZHOU Ji, FENG Shan Shan. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice. Biomedical and Environmental Sciences, 2018, 31(10): 757-768.
      样品类型: Plasma,Tissue homogenate
    6. 文献: Ren F, Li J, Wang Y, et al. The Effects of Angelica Sinensis Polysaccharide on Tumor Growth and Iron Metabolism by Regulating Hepcidin in Tumor-Bearing Mice[J]. Cellular Physiology and Biochemistry, 2018, 47(3): 1084-1094.
      样品类型: Serum
    7. 文献: Wang J, Wang L, Zhou J, et al. The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice[J]. Biomedicine & Pharmacotherapy, 2018, 106: 1250-1257.
      样品类型: Serum,Tissue homogenate
    8. 文献: Deng Z, Li Z, Sun C, et al. The Association Between Inflammation, The Microbiome and Urethane-induced Pulmonary Adenocarcinoma[J]. Oncology Letters, 2018, 15(5): 6352-6360.
    9. 文献: Zhang Z, Zhang H, Chen R, et al. Oral supplementation with ursolic acid ameliorates sepsis-induced acute kidney injury in a mouse model by inhibiting oxidative stress and inflammatory responses[J]. Molecular Medicine Reports, 2018, 17(5): 7142-7148.
    10. 文献: Rao S S, Hu Y, Xie P L, et al. Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines[J]. Bone research, 2018, 6(1): 9.
    11. 文献: Wang Y M, Liu Z Y, Ai Y H, et al. Blocking The CD38/cADPR Pathway Plays A Double-edged Role In LPS Stimulated Microglia[J]. Neuroscience, 2017, 361: 34-42.
      样品类型: Cell culture supernatant
    12. 文献: Wang F, Peng P L, Lin X, et al. Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis[J]. Oncotarget, 2017, 8(58): 98945-98952.
      样品类型: Tissue homogenate
    13. 文献: Cai Y, Sun W, Zhang X X, et al. Osthole Prevents Acetaminophen-Induced Liver Injury In Mice[J]. Acta Pharmacologica Sinica, 2017.
      样品类型: Tissue homogenate
    14. 文献: Chen L, Zhou Z Y, Yang Y, et al. Therapeutic Effect Of imiquimod On Dextran Sulfate Sodium-induced Ulcerative Colitis In Mice[J]. Plos One, 2017.
      样品类型: Serum
    15. 文献: Valdor R, García-Bernal D, Bueno C, et al. Glioblastoma Progression Is Assisted By Induction Of Immunosuppressive Function Of Pericytes Through Interaction With Tumor Cells[J]. Oncotarget, 2017, 8(40): 68614-68626.
      样品类型: Cell culture supernatant
    16. 文献: Zhang M, Zhao H T, Cai J, et al. Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet[J]. PloS one, 2017.
      样品类型: Serum
    17. 文献: Li H D, Chen X, Yang Y, et al. Wogonin Attenuates Inflammation By Activating PPAR-γ In Alcoholic Liver Disease[J]. International Immunopharmacology, 2017, 50: 95-106.
      样品类型: Serum,Cell culture supernatant
    18. 文献: Aipire A, Li J, Yuan P, et al. Glycyrrhiza Uralensis Water Extract Enhances Dendritic Cell Maturation And Antitumor Efficacy Of HPV Dendritic Cell-Based Vaccine[J]. Scientific Reports, 2017.
      样品类型: Cell culture supernatant
    19. 文献: Hu M, Luo Q, Alitongbieke G, et al. Celastrol-Induced Nur77 Interaction With TRAF2 Alleviates Inflammation By Promoting Mitochondrial Ubiquitination And Autophagy[J]. Molecular Cell, 2017, 66(1): 141-153.
      样品类型: Serum
    20. 文献: Hamuti A, Li J Y, Zhou F F,et al. Capparis Spinosa Fruit Ethanol Extracts Exert Different Effects On The Maturation Of Dendritic Cells[J]. Molecules, 2017.
      样品类型: Cell culture supernatant
    21. 文献: Wu D D, Gao B, Li M L, et al. Hydrogen Sulfide Mitigates Kidney Injury In High Fat Diet-Induced Obese Mice[J]. Oxidative Medicine And Cellular Longevity, 2016.
      样品类型: Plasma
    22. 文献: Wang J, Ge B, Li Z, et al. Structural Analysis And Immunoregulation Activity Comparison Of Five Polysaccharides From Angelica Sinensis[J]. Carbohydrate Polymers, 2016, 140: 6-12.
      样品类型: Cell culture supernatant
    23. 文献: Wan G, Lingao R, Yu Y, et al. Effects Of Silver Nanoparticles In Combination With Antibiotics On The Resistant Bacteria Acinetobacter Baumannii[J]. International Journal of Nanomedicine, 2016, 11: 3789-3800.
      样品类型: Plasma
    24. 文献: Hao S, Li H, Sun X, et al. An Unusual Case Of Iron Deficiency Anemia Is Associated With Extremely Low Level Of Transferrin Receptor[J]. International Journal of Clinical and Experimental Pathology, 2015, 8(7): 8613-8618.
      样品类型: Serum
    25. 文献: Wang J, Ge B, Du C, et al. Sulfated Modification Promotes The Immunomodulatory Bioactivities Of Lyciumbarbarum Polysaccharides In Vitro[J]. International Journal of Clinical and Experimental Medicine, 2015, 8(11): 20380-20390.
      样品类型: Cell culture supernatant
    26. 文献: Yan Q, Xiang Y, Shuyu W, et al. PGC-1α Silencing Compounds The Perturbation Of Mitochondrial Function Caused By Mutant SOD1 In Skeletal Muscle Of ALS Mouse Model[J]. Frontiers in Aging Neuroscience, 2015.
      样品类型: Serum
    27. 文献: Chen L, Shen X, Chen G, et al. A Comparative Study Of The Effects Upon LPS Induced Macrophage RAW264.7 Inflammation In Vitro Of The Lipids Of Hippocampus Trimaculatus Leach[J]. Journal of Oleo Science, 2015, 64(12): 1273-1281.
      样品类型: Cell culture supernatant
    28. 文献: Cheng J, Zhang L, Dai W, et al. Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B[J]. Biochemical & Biophysical Research Communications, 2015, 458(1): 140-147.
      样品类型: Tissue homogenate
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