Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称:小鼠基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒
MMP9、CLG4B、Gelatinase B、GELB、MANDP2、92kDa Type IV Collagenase、92 KDa Gelatinase
Price:
- 反应性: Mouse
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠MMP-9抗体包被于酶标板上,实验时样品(或标准品)中的小鼠MMP-9会与包被抗体结合。后依次加入生物素化的抗小鼠MMP-9抗体和辣根过氧化物酶标记的亲和素,抗小鼠MMP-9抗体与结合在包被抗体上的小鼠MMP-9结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,MMP-9浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MMP-9的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠MMP-9,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Quantitative phosphoproteomic analysis identifies the critical role of JNK1 in neuroinflammation induced by Japanese encephalitis virus
IF: 7.359Journal:Science Signaling -
G Protein-Coupled Estrogen Receptor 30 Reduces Transverse Aortic Constriction-Induced Myocardial Fibrosis in Aged Female Mice by Inhibiting the ERK1/2 -MMP-9 Signaling Pathway.
IF: 5.811Journal:Frontiers in Pharmacology -
Twelve novel sesquiterpenes with anti-inflammatory and cholesterol-lowering activities from burdock leaves
IF: 5.275 -
pNaKtide ameliorates renal interstitial fibrosis through inhibition of sodium-potassium adenosine triphosphatase-mediated signaling pathways in unilateral ureteral obstruction mice
IF: 4.198Journal:NEPHROLOGY DIALYSIS TRANSPLANTATION -
Expression of Matrix Metalloproteinases and Tissue Inhibitor of Matrix Metalloproteinases during Apical Periodontitis Development
IF: 4.171 -
Arbutin protects brain against middle cerebral artery occlusion-reperfusion (MCAo/R) injury
IF: 3.575Journal:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONSPMID:34507065 -
Microcyst fluid promotes the migration and invasion of fibroblasts in the adventitial layer of alveolar echinococcosis
IF: 3.112 -
Marsdenia tenacissima extract suppresses tumor growth and angiogenesis in A20 mouse lymphoma
IF: 2.967Journal:Oncology Letters -
Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway
IF: 2.63Journal:Evidence-based Complementary and Alternative MedicinePMID:35178108 -
Association of PAR-2 Gene Polymorphisms with the Inflammatory Response and Susceptibility to Knee Osteoarthritis in the Chinese Han Population
IF: 1.121
Q1:Can ELISA measure the cell supernatant MMP-2 and MMP-9? I read the papers written by others, in addition to measuring the expression of these two indicators, but also to measure the activity, can ELISA measure the activity? What's the difference with gelatin enzymology?
These two kits can be used for the detection of your cell supernatant. However, ELISA detection of cell supernatants is affected by factors such as media components, cell growth, and external drug stimulation conditions, and it is recommended that you conduct a preliminary experiment before the formal experiment. Gelatin enzyme spectrometry uses the reversible combination of SDS and MMPs in the sample to separate MMP-2 and MMP-9 in the sample, and then restores the activity of both through the bivalent metal ion buffer system. This test method is specifically aimed at the activity detection of MMP-2 and MMP-9. ELISA detects the content (that is, concentration) of the tested substance through the binding reaction of antigen and antibody, that is, captures the MMP-2 and MMP-9 of the sample through the specific antibodies of MMP-2 and MMP-9. The experimental principles of the two are different, and the activity of MMP-2 and MMP-9 cannot be detected by ELISA.