N/H/K-Ras Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
- 宿主： Rabbit
- 反应性： Mouse;Rat
- 应用性： WB
| Verified Samples |
Verified Samples in WB:Mouse lung,Mouse colon,Mouse kidney,Mouse brain,Rat lung,Rat colon,Rat kidney,Rat brain |
| Dilution | WB 1:500-1:2000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse K-Ras |
| Abbre | N/H/K-Ras |
| Synonyms | NRAS;HRAS1;GTPase NRas;Transforming protein N-Ras;HRAS;HRAS1;GTPase HRas;H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras;KRAS;KRAS2;RASK2;GTPase KRas;K-Ras 2;Ki-Ras;c-K-ras;c-Ki-ras抗体 |
| Swissprot | |
| Calculated MW | 21kDa |
| Observed MW | 21kDa |
| Cellular Localization | Cell membrane, Cytoplasm, Membrane |
| Concentration | 0.58 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling; Signal Transduction |
| Conjugation | Unconjugated |
| Background | NRAS an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. |
Western Blot analysis of various samples using N/H/K-Ras Polyclonal Antibody at dilution of 1:800.
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
