NFAT2 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Jurkat,U87-MG,K562 Verified Samples in IHC:Rat brain,Human tonsil,Mouse brain,Human placenta |
| Dilution | WB 1:500-1:3000;IHC 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein of human NFAT2 (NP_765978.1). |
| Abbre | NFAT2 |
| Synonyms | NF-ATC;NF-ATc1.2;NFAT2;NFATc;NFATC1抗体 |
| Swissprot | |
| Calculated MW | 38kDa/74- 88kDa/100-101kDa |
| Observed MW | 78kDa |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. |
| Concentration | 1mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Immunology; Signal Transduction |
| Conjugation | Unconjugated |
| Background | The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. |
Western blot analysis of extracts of various cell lines using NFAT2 Polyclonal Antibody at dilution of 1:3000.
Immunohistochemistry of paraffin-embedded Rat brain using NFAT2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human tonsil using NFAT2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using NFAT2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human placenta using NFAT2 Polyclonal Antibody at dilution of 1:200 (40x lens).
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
