Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit
游离脂肪酸(NEFA/FFA)比色法测试盒
| 规格： | / 48T(16 samples) / 96T(40 samples) |
| 价格： | / ￥480 / ￥860 |
货号：E-BC-K013-M
检测仪器及波长: 酶标仪（690-730 nm，最佳波长715 nm）
样本类型: 动物组织
检测原理
在弱酸性条件下，游离脂肪酸与铜盐反应生成铜皂，在715 nm处有特征吸收峰，在一定范围内游离脂肪酸含量与显色程度呈线性关系，可计算出游离脂肪酸的含量。
关键信息
| 指标简称 | NEFA/FFA |
| 样本类型 | 动物组织 |
| 灵敏度 | 0.15 mmol/L |
| 检测范围 | 0.15-1.5 mmol/L |
| 精密度 | 批间差：5.1%批内差：3.3% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，根据预实验的结果，结合本试剂盒的线性范围（0.15-1.5 mmol/L），请参考下表稀释：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
大鼠肝组织匀浆抽提液 |
不稀释 |
大鼠肾组织匀浆抽提液 |
不稀释 |
|
大鼠心组织匀浆抽提液 |
不稀释 |
小鼠肝组织匀浆抽提液 |
不稀释 |
注：稀释液为试剂一
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