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NRAS Polyclonal Antibody

Price: ¥1640 ¥1010

Size:
100μL 50μL
  • 宿主: Rabbit
  • 反应性: Human;Mouse;Rat
  • 应用性: WB
Verified Samples
Verified Samples in WB:Human Jurkat,Human 293T,Mouse NIH/3T3,Rat PC-12 ,Rat ovary
Dilution
WB 1:500-1:1000;IF 1:50-1:200
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant Human NRAS protein expressed by E.coli
Abbre
NRAS
Synonyms
ALPS4;AV095280;GTPase NRas;HRAS1;N ras;N ras protein part 4;Neuroblastoma RAS viral (v ras) oncogene homolog;NRAS;NRAS1;NS6;OTTHUMP00000013879;OTTMUSP00000023521;RASN;Transforming protein N Ras;Transforming protein N-Ras;v ras neuroblastoma RAS viral oncogene homolog
Swissprot
Calculated MW
21kDa
Observed MW
21kDa
Concentration
1 mg/mL
Storage
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH 7.4
Purification Method
Affinity purification
Research Areas
Cancer; Signal Transduction
Conjugation
Unconjugated
Background
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. 
Western blot with NRAS Polyclonal antibody at dilution of 1:1000.lane 1:Jurkat whole cell lysate,lane 2:293T whole cell lysate,lane3:NIH/3T3 whole cell lysate,lane4:PC-12 whole cell lysate,lane5:Rat ovary
E-AB-1003 Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1194 HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays


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