One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)
一步法TUNEL原位细胞凋亡检测试剂盒(绿色,Elab Fluor® 488)
规格: | / 20Assays / 50Assays / 100Assays |
价格: | / ¥1500 / ¥2000 / ¥3600 |
货号:E-CK-A321
检测原理
细胞在发生凋亡时,会激活一些特异性的DNA内切酶,这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段,在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶(脱氧核糖核酸末端转移酶)将标记的dUTP连接到断裂DNA暴露的3'-OH末端,通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞,从而可以通过荧光显微镜进行检测。
基本信息
应用类型 | 细胞凋亡-DNA片段化 |
检测方法 | 荧光 |
样本类型 | 石蜡切片,冰冻切片,细胞爬片,细胞涂片 |
检测时间 | 3 hours |
检测仪器 | 荧光显微镜 |
荧光 | Elab Fluor® 488 |
Ex/Em (nm) | 495/519 |
Channel set | FITC |
Filter set | FITC |
自备试剂 | 4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇 |
保存温度 | -20°C, shading light |
运输条件 | 冰袋 |
保质期 | 12 months |
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