One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
一步法TUNEL原位细胞凋亡检测试剂盒（绿色，FITC）
| 规格： | / 20Assays / 50Assays / 100Assays |
| 价格： | / ￥1500 / ￥2000 / ￥3600 |
货号：E-CK-A320
检测原理
细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。
基本信息
| 应用类型 | 细胞凋亡-DNA片段化 |
| 检测方法 | 荧光 |
| 样本类型 | 石蜡切片，冰冻切片，细胞爬片，细胞涂片 |
| 检测时间 | 3 hours |
| 检测仪器 | 荧光显微镜 |
| 荧光 | FITC |
| Ex/Em (nm) | 490/520 |
| Channel set | FITC |
| Filter set | FITC |
| 自备试剂 | 4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇 |
| 保存温度 | -20°C, shading light |
| 运输条件 | 冰袋 |
| 保质期 | 12 months |
操作指南
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