本公司产品仅供体外研究使用,不用于临床诊断

One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

一步法TUNEL原位细胞凋亡检测试剂盒(绿色,Elab Fluor® 488)

规格: / 20Assays / 50Assays / 100Assays
价格: / ¥1500 / ¥2000 / ¥3600

货号:E-CK-A321

  • 详情
  • 文献(19)
  • 检测原理

    细胞在发生凋亡时,会激活一些特异性的DNA内切酶,这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段,在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶(脱氧核糖核酸末端转移酶)将标记的dUTP连接到断裂DNA暴露的3'-OH末端,通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞,从而可以通过荧光显微镜进行检测。

    基本信息

    应用类型 细胞凋亡-DNA片段化
    检测方法 荧光
    样本类型 石蜡切片,冰冻切片,细胞爬片,细胞涂片
    检测时间 3 hours
    检测仪器 荧光显微镜
    荧光 Elab Fluor® 488
    Ex/Em (nm) 495/519
    Channel set FITC
    Filter set FITC
    自备试剂 4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇
    保存温度 -20°C, shading light
    运输条件 冰袋
    保质期 12 months

    操作指南

    客户发表文献

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