Phospho-SMAD3 (Ser425) Monoclonal Antibody
Price:
Size:
120μL
60μL
20μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： IHC-p
| Verified Samples |
Verified Samples in IHC:Human skin |
| Dilution | IHC 1:50-300 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide of Phospho-Smad3 (Ser425) |
| Abbre | Phospho-SMAD3 (Ser425) |
| Synonyms | DKFZP586N0721;DKFZp686J10186;hMAD 3;hMAD-3;hSMAD3;HSPC193;HST17436;JV15 2;JV15-2;JV152;LDS1C;LDS3;MAD (mothers against decapentaplegic Drosophila) homolog 3;MAD homolog 3;Mad homolog JV15 2;Mad protein homolog;MAD;mothers against decapentaplegic homolog 3;Mad3;MADH 3;MADH3;MGC60396;Mothers against decapentaplegic homolog 3;Mothers against DPP homolog 3;SMA and MAD related protein 3;SMAD 3;SMAD;SMAD family member 3;SMAD;mothers against DPP homolog 3;Smad3;SMAD3抗体 |
| Swissprot | |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification Method | Protein A purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling; Metabolism; Signal Transduction; Stem Cells |
| Clone No. | Clone:4C5 |
| Conjugation | Unconjugated |
| Background | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
Immunohistochemistry of paraffin-embedded Human skin tissue using Phospho-SMAD3 (Ser425) Monoclonal Antibody at dilution of 1:200
| E-AB-1001 | Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1024 | Goat Anti-Mouse IgG(H+L)(Biotin conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1196 | HRP-Goat Anti-Mouse IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
