PLEKHO1 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Hela,Mouse muscle,Mouse kidney Verified Samples in IHC:Human thyroid cancer,Human cervical cancer |
| Dilution | WB 1:200-1:1000;IHC 1:100-1:300 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human PLEKHO1 |
| Abbre | PLEKHO1 |
| Synonyms | C-Jun-binding protein;Casein kinase 2-interacting protein 1;CK2 interacting protein 1;CK2 interacting protein 1 HQ0024c protein;CK2-interacting protein 1;CKIP 1;CKIP-1;CKIP1;JBP;OC120;Osteoclast maturation associated gene 120 protein;Osteoclast maturation-associated gene 120 protein;PH domain-containing family O member 1;PKHO1;Pleckstrin homology domain containing family O member 1;Pleckstrin homology domain-containing family O member 1;PLEKHO1抗体 |
| Swissprot | |
| Calculated MW | 46kDa |
| Cellular Localization | Cell membrane. Nucleus. Cytoplasm. Predominantly localized to the plasma membrane. In C2C12 cells, with the absence of growth factor, it is found in the nucleus. It rapidly translocates to the plasma membrane after insulin stimulation. In response to TNF, it translocates from the plasma membrane to the cytoplasm and then to the nucleus accompanied by cleavage by caspase-3. However, the subcellular location is highly dependent of the cell type, and this explains why it is found exclusively at the plasma membrane, in some type of cells. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Signal Transduction |
| Conjugation | Unconjugated |
| Background | Pleckstrin homology domain-containing family O member 1is aproteinthat in humans is encoded by thePLEKHO1gene. Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. |
Western Blot analysis of Hela cell, Mouse muscle and kidney tissue using PLEKHO1 Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human thyroid cancer using PLEKHO1 Polyclonal Antibody at dilution of 1:70
Immunohistochemistry of paraffin-embedded Human cervical cancer using PLEKHO1 Polyclonal Antibody at dilution of 1:70
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
