Potassium (K) Turbidimetric Assay Kit
钾离子(K)比浊法测试盒
规格: | / 48T(32 samples) / 96T(80 samples) |
价格: | / ¥280 / ¥500 |
货号:E-BC-K279-M
检测仪器及波长: 酶标仪(450 nm-600nm,最佳检测波长为450 nm)
样本类型: 血清(浆);乳汁;动物组织;细胞及细胞上清
检测原理
在碱性条件下,四苯基硼酸钠与待测液中的钾离子反应,形成溶解度小的四苯基硼酸钾白色细小颗粒,在溶液中呈悬浮状态,形成浊液,在一定范围内与钾离子浓度成正比。
关键信息
指标简称 | K |
样本类型 | 血清(浆);乳汁;动物组织;细胞及细胞上清 |
灵敏度 | 0.002 mmol/L |
检测范围 | 0.01-0.80 mmol/L |
精密度 | 批间差:6.1%批内差:1.1% |
检测仪器及波长 | 酶标仪 |
保存条件 | 2-8℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择2-3个预期差异大的样本稀释成不同浓度进行预实验,结合本试剂盒的线性范围(0.01-0.80 mmol/L),选取最佳稀释倍数,进行正式批量实验。不同样本的稀释比例范围如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
人血清 |
不稀释 |
人血浆 |
不稀释 |
大鼠血清 |
不稀释 |
人乳汁 |
不稀释 |
RAW264.7细胞上清 |
不稀释 |
10%大鼠肝匀浆 |
2-4 |
注:稀释液为双蒸水。
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