QuicKey Human CRP(C-Reactive Protein) ELISA Kit
QuicKey-人C反应蛋白(CRP)酶联免疫吸附测定试剂盒
| 规格： | / 96T / 48T / 96T*5 |
| 价格： | / ￥2000 / ￥1400 / ￥9000 |
货号：E-TSEL-H0007
货期：3个工作日+顺丰周期
反应性: Human
检测范围: 0.39~25 ng/mL
灵敏度: 0.23 ng/mL
产品信息
QuicKey系列
与传统ELISA试剂盒相比，在实验时间节省至少1小时的同时，获得更加灵敏和精确的实验结果。Elabscience 自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。
基础参数
| 反应类型 | 双抗体夹心法ELISA |
| 规格 | 96T/48T |
| 反应时间 | 2.5 h |
| 反应性 | 人 |
| 检测方法 | 比色法 |
| 检测范围 | 0.39-25 ng/mL |
| 灵敏度 | 0.23 ng/mL |
| 上样体积 | 50 μL/孔 |
| 样本类型 | 血清，血浆 |
| 特异性 | 可检测样本中的人 CRP , 且与其它类似物无明显交叉反应。 |
| 重复性 | 板内，板间变异系数均<10%。 |
| 用途 | 该试剂盒用于体外定量检测人 血清，血浆中CRP浓度。其它相关生物液体请咨询技术支持。 |
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗人 CRP抗体包被于酶标板上，实验时样品（或标准品）及生物素化的抗人 CRP抗体同时加入酶标板中，样本（或标准品）中的人 CRP会与包被抗体结合，同时抗人 CRP抗体与结合在包被抗体上的人 CRP结合，游离的成分被洗去。再加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，CRP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CRP的浓度。
试剂盒组分及保存
未拆封的试剂盒可在2-8℃ 保存6个月；试剂盒组分开封后请按照下表中的条件分别保存。
| 中文名称 | 规格 | 开封后保存条件 |
|---|---|---|
| ELISA 酶标板 |
96T：8 孔×12 条 48T：8 孔×6 条 |
|
| 冻干标准品 |
96T：2 支 48T：1 支 |
2-8℃，复溶后当天使用 |
| 标准品&样品稀释液 | 1瓶 20mL | 2-8℃ |
| 生物素化抗体工作液 | 1瓶 6mL | |
| 酶结合物稀释液 | 1瓶 14mL | |
| 浓缩洗涤液 | 1瓶 30mL | |
| 浓缩HRP酶结合物（100×） |
96T：1支 120μL 48T：1支 60μL |
2-8℃ (避光) |
| 底物溶液(TMB) | 1瓶 10mL | |
| 反应终止液 | 1瓶 10mL | 2-8℃ |
| 封板覆膜 | 5张 | |
| 产品说明书 | 1 份 | |
| 质检报告 | 1 份 |
说明：所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。试剂体积以实际发货版说明书为准。 相关试剂在分装时会比标签上标明的体积稍多一些，请在使用时量取而非直接倒出。
试验所需自备物品
1. 酶标仪(450nm波长滤光片)
2. 高精度移液器，EP管及一次性吸头：0.5-10μL, 2-20μL, 20-200μL, 200-1000μL
3. 37℃恒温箱，双蒸水或去离子水
4. 吸水纸
技术数据
样本测值参考
标曲示例
以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线 。
| 浓度：ng/mL | 25 | 12.5 | 6.25 | 3.13 | 1.56 | 0.78 | 0.39 | 0 |
| OD | 2.434 | 1.587 | 1.027 | 0.607 | 0.353 | 0.211 | 0.134 | 0.068 |
| 校正OD | 2.366 | 1.519 | 0.959 | 0.539 | 0.285 | 0.143 | 0.066 | - |
精密度
板内精密度：低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度：低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| 批内变异系数 | 批间变异系数 | |||||
| 样本 | 1 | 2 | 3 | 1 | 2 | 3 |
| 数量 | 20 | 20 | 20 | 20 | 20 | 20 |
| 平均值(ng/mL) | 1.19 | 3.11 | 11.37 | 1.18 | 2.73 | 11.41 |
| 标准差 | 0.07 | 0.15 | 0.55 | 0.06 | 0.13 | 0.51 |
| 变异系数 (%) | 5.88 | 4.82 | 4.84 | 5.08 | 4.76 | 4.47 |
回收率
分别往不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率 。
| 样本类型 | 回收率范围 (%) | 平均回收率 (%) |
| 血清(n=8) | 95-103 | 98 |
| 血浆(EDTA)(n=8) | 95-108 | 103 |
线性
分别往4个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将4个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率 。
| 血清(n=4) | 血浆(EDTA) (n=4) | ||
| 1:2 | 回收率范围(%) | 91-103 | 94-103 |
| 平均回收率(%) | 96 | 98 | |
| 1:4 | 回收率范围(%) | 95-105 | 98-109 |
| 平均回收率(%) | 99 | 102 | |
| 1:8 | 回收率范围(%) | 91-104 | 89-96 |
| 平均回收率(%) | 99 | 92 | |
| 1:16 | 回收率范围(%) | 95-105 | 83-96 |
| 平均回收率(%) | 99 | 91 |
文献引用
- 文献: Post-COVID-19 Patients Who Develop Lung Fibrotic-like Changes Have Lower Circulating Levels of IFN-β but Higher Levels of IL-1α and TGF-β
研究种属: Human
样品类型: plasma - 文献: Differential levels of inflammatory and neuroendocrine markers in the hippocampus and anterior cingulate cortex of bipolar disorder subjects: A post-mortem study
研究种属: Human
样品类型: Tissue homogenate - 文献: Relationship between the Dietary Inflammatory Index Score and Cytokine Levels in Chinese Pregnant Women during the Second and Third Trimesters
研究种属: Human
样品类型: serum - 文献: A plasmonic ELISA for multi-colorimetric sensing of C-reactive protein by using shell dependent etching of Ag coated Au nanobipyramids
样品类型: AuNBP@Ag - 文献: Clinical, inflammatory and microbiological outcomes of full‐month scaling with adjunctive glycine powder air‐polishing. A randomized trial
研究种属: Human
样品类型: Serum,Gingival Crevicular Fluid - 文献: Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis
研究种属: Human
样品类型: serum - 文献: Effect of fructooligosaccharide on endothelial function in CKD patients: a randomized controlled trial
研究种属: Human
样品类型: serum - 文献: Changes in plasma fatty acids and related biomarkers during transition to an exclusively plant- and fish-based diet in healthy adults
研究种属: Human
样品类型: Plasma - 文献: Obesity-related proteins score as a potential marker of breast cancer risk
研究种属: Human
样品类型: Plasma - 文献: Intermittent Fasting Enhanced the Cognitive Function in Older Adults with Mild Cognitive Impairment by Inducing Biochemical and Metabolic changes: A 3-Year Progressive Study
研究种属: Human
样品类型: Plasma - 文献: Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression
研究种属: Human
样品类型: serum - 文献: TSG attenuates LPC‐induced endothelial cells inflammatory damage through notch signaling inhibition
研究种属: Human
样品类型: Cell culture supernatant - 文献: Faecal but not serum calprotectin levels look promising in predicting active disease in Behçet's syndrome patients with gastrointestinal involvement.
研究种属: Human
样品类型: stool - 文献: Prognostic Significance of Serum NGAL and Troponin I against Acute Kidney Injury in Egyptian ICU Patients after Open Heart Surgery: A Pilot Study
研究种属: Human
样品类型: Serum - 文献: Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes
研究种属: Human
样品类型: Serum - 文献: Changes of biochemical biomarkers in the serum of children with convulsion status epilepticus: a prospective study
研究种属: Human
样品类型: serum - 文献: The effects of blood purification combined with antibiotics on extravascular lung water index, inflammatory factors, and prognosis of patients with severe acute pancreatitis complicated with acute respiratory distress syndrome.
研究种属: Human
样品类型: blood - 文献: High anti-human cytomegalovirus antibody levels are associated with the progression of essential hypertension and target organ damage in Han Chinese population
研究种属: Human
样品类型: Serum - 文献: Sex difference in IL-6 modulation of cognition among Chinese individuals with major depressive disorder
研究种属: Human
样品类型: Serum - 文献: A comparison of effects of scalp nerve block and local anesthetic infiltration on inflammatory response, hemodynamic response, and postoperative pain in patients undergoing craniotomy for cerebral aneurysms: a randomized controlled trial
研究种属: Human
样品类型: plasm - 文献: Incremental Accuracy of Blood Biomarkers for Predicting Clinical Outcomes After Intracerebral Hemorrhage
研究种属: Human
样品类型: serum - 文献: A comparative study of autologous rectus fascia pubovaginal sling surgery and synthetic transobturator vaginal tape procedure in treatment of women with urodynamic stress urinary incontinence
研究种属: Human
样品类型: blood - 文献: The effects of aerobic exercise and sertraline on pro-inflammatory indices and amelioration of neurotrophic factors in patients who have undergone CABG
研究种属: Human
样品类型: plasma
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实验操作
| 1. 对应板孔中加入50μL标准品工作液或样本后，立即每孔加入50μL生物素化抗体工作液，37°C孵育90分钟 | |
| 2. 弃掉板内液体，洗板3次 | |
| 3. 每孔加入100μL HRP酶结合物工作液，37°C孵育30分钟，弃掉板内液体，洗板5次 | |
| 4. 每孔加入90μL底物溶液，37°C孵育15分钟左右 | |
| 5. 每孔加入50μL终止液 | |
| 6. 立即在450nm波长下读数，处理数据 |
客户发表文献
- 文献: Post-COVID-19 Patients Who Develop Lung Fibrotic-like Changes Have Lower Circulating Levels of IFN-β but Higher Levels of IL-1α and TGF-β
样品类型: plasma - 文献: Differential levels of inflammatory and neuroendocrine markers in the hippocampus and anterior cingulate cortex of bipolar disorder subjects: A post-mortem study
样品类型: Tissue homogenate - 文献: Relationship between the Dietary Inflammatory Index Score and Cytokine Levels in Chinese Pregnant Women during the Second and Third Trimesters
样品类型: serum - 文献: A plasmonic ELISA for multi-colorimetric sensing of C-reactive protein by using shell dependent etching of Ag coated Au nanobipyramids
样品类型: AuNBP@Ag - 文献: Clinical, inflammatory and microbiological outcomes of full‐month scaling with adjunctive glycine powder air‐polishing. A randomized trial
样品类型: Serum,Gingival Crevicular Fluid - 文献: Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis
样品类型: serum - 文献: Effect of fructooligosaccharide on endothelial function in CKD patients: a randomized controlled trial
样品类型: serum - 文献: Changes in plasma fatty acids and related biomarkers during transition to an exclusively plant- and fish-based diet in healthy adults
样品类型: Plasma - 文献: Obesity-related proteins score as a potential marker of breast cancer risk
样品类型: Plasma - 文献: Intermittent Fasting Enhanced the Cognitive Function in Older Adults with Mild Cognitive Impairment by Inducing Biochemical and Metabolic changes: A 3-Year Progressive Study
样品类型: Plasma - 文献: Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression
样品类型: serum - 文献: TSG attenuates LPC‐induced endothelial cells inflammatory damage through notch signaling inhibition
样品类型: Cell culture supernatant - 文献: Faecal but not serum calprotectin levels look promising in predicting active disease in Behçet's syndrome patients with gastrointestinal involvement.
样品类型: stool - 文献: Prognostic Significance of Serum NGAL and Troponin I against Acute Kidney Injury in Egyptian ICU Patients after Open Heart Surgery: A Pilot Study
样品类型: Serum - 文献: Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes
样品类型: Serum - 文献: Changes of biochemical biomarkers in the serum of children with convulsion status epilepticus: a prospective study
样品类型: serum - 文献: The effects of blood purification combined with antibiotics on extravascular lung water index, inflammatory factors, and prognosis of patients with severe acute pancreatitis complicated with acute respiratory distress syndrome.
样品类型: blood - 文献: High anti-human cytomegalovirus antibody levels are associated with the progression of essential hypertension and target organ damage in Han Chinese population
样品类型: Serum - 文献: Sex difference in IL-6 modulation of cognition among Chinese individuals with major depressive disorder
样品类型: Serum - 文献: A comparison of effects of scalp nerve block and local anesthetic infiltration on inflammatory response, hemodynamic response, and postoperative pain in patients undergoing craniotomy for cerebral aneurysms: a randomized controlled trial
样品类型: plasm - 文献: Incremental Accuracy of Blood Biomarkers for Predicting Clinical Outcomes After Intracerebral Hemorrhage
样品类型: serum - 文献: A comparative study of autologous rectus fascia pubovaginal sling surgery and synthetic transobturator vaginal tape procedure in treatment of women with urodynamic stress urinary incontinence
样品类型: blood - 文献: The effects of aerobic exercise and sertraline on pro-inflammatory indices and amelioration of neurotrophic factors in patients who have undergone CABG
样品类型: plasma
