QuicKey Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
QuicKey-人中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫吸附测定试剂盒
规格: | / 96T / 48T / 96T*5 |
价格: | / ¥2600 / ¥1800 / ¥11700 |
货号:E-TSEL-H0003
货期:3个工作日+顺丰周期
反应性: Human
检测范围: 0.13~8 ng/mL
灵敏度: 0.06 ng/mL
产品信息
QuicKey系列
与传统ELISA试剂盒相比,在实验时间节省至少1小时的同时,获得更加灵敏和精确的实验结果。Elabscience 自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。
基础参数
反应类型 | 双抗体夹心法ELISA |
规格 | 96T/48T |
反应时间 | 2.5 h |
反应性 | 人 |
检测方法 | 比色法 |
检测范围 | 0.13-8 ng/mL |
灵敏度 | 0.06 ng/mL |
上样体积 | 50 μL/孔 |
样本类型 | 血清,血浆,尿液 |
特异性 | 可检测样本中的人 NGAL , 且与其它类似物无明显交叉反应。 |
重复性 | 板内,板间变异系数均<10%。 |
用途 | 该试剂盒用于体外定量检测人 血清,血浆,尿液中NGAL浓度。其它相关生物液体请咨询技术支持。 |
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗人 NGAL抗体包被于酶标板上,实验时样品(或标准品)及生物素化的抗人 NGAL抗体同时加入酶标板中,样本(或标准品)中的人 NGAL会与包被抗体结合,同时抗人 NGAL抗体与结合在包被抗体上的人 NGAL结合,游离的成分被洗去。再加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,NGAL浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中NGAL的浓度。
试剂盒组分及保存
未拆封的试剂盒可在2-8℃ 保存6个月;试剂盒组分开封后请按照下表中的条件分别保存。
中文名称 | 规格 | 开封后保存条件 |
---|---|---|
ELISA 酶标板 |
96T:8 孔×12 条 48T:8 孔×6 条 |
|
冻干标准品 |
96T:2 支 48T:1 支 |
2-8℃,复溶后当天使用 |
标准品&样品稀释液 | 1瓶 20mL | 2-8℃ |
生物素化抗体工作液 | 1瓶 6mL | |
酶结合物稀释液 | 1瓶 14mL | |
浓缩洗涤液 | 1瓶 30mL | |
浓缩HRP酶结合物(100×) |
96T:1支 120μL 48T:1支 60μL |
2-8℃ (避光) |
底物溶液(TMB) | 1瓶 10mL | |
反应终止液 | 1瓶 10mL | 2-8℃ |
封板覆膜 | 5张 | |
产品说明书 | 1 份 | |
质检报告 | 1 份 |
说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。试剂体积以实际发货版说明书为准。 相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。
试验所需自备物品
1. 酶标仪(450nm波长滤光片)
2. 高精度移液器,EP管及一次性吸头:0.5-10μL, 2-20μL, 20-200μL, 200-1000μL
3. 37℃恒温箱,双蒸水或去离子水
4. 吸水纸
技术数据
样本测值参考
标曲示例
以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线 。
浓度:ng/mL | 8 | 4 | 2 | 1 | 0.5 | 0.25 | 0.13 | 0 |
OD | 2.569 | 2.13 | 1.533 | 1.088 | 0.656 | 0.437 | 0.225 | 0.059 |
校正OD | 2.51 | 2.071 | 1.474 | 1.029 | 0.597 | 0.378 | 0.166 | - |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
批内变异系数 | 批间变异系数 | |||||
样本 | 1 | 2 | 3 | 1 | 2 | 3 |
数量 | 20 | 20 | 20 | 20 | 20 | 20 |
平均值(ng/mL) | 0.37 | 0.90 | 3.57 | 0.35 | 0.81 | 3.73 |
标准差 | 0.02 | 0.04 | 0.16 | 0.02 | 0.04 | 0.17 |
变异系数 (%) | 5.41 | 4.44 | 4.48 | 5.71 | 4.94 | 4.56 |
回收率
分别往不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率 。
样本类型 | 回收率范围 (%) | 平均回收率 (%) |
血清(n=8) | 86-100 | 94 |
血浆(EDTA)(n=8) | 101-110 | 104 |
尿液(n=8) | 87-98 | 90 |
线性
分别往4个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将4个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率 。
血清(n=4) | 血浆(EDTA) (n=4) | 尿液(n=4) | ||
1:2 | 回收率范围(%) | 88-99 | 90-101 | 101-110 |
平均回收率(%) | 91 | 96 | 104 | |
1:4 | 回收率范围(%) | 95-105 | 92-102 | 96-109 |
平均回收率(%) | 99 | 97 | 102 | |
1:8 | 回收率范围(%) | 87-96 | 86-100 | 98-111 |
平均回收率(%) | 92 | 93 | 105 | |
1:16 | 回收率范围(%) | 101-111 | 90-98 | 100-110 |
平均回收率(%) | 105 | 95 | 104 |
靶标信息
文献引用
- 文献: Identification of key candidate biomarkers for severe influenza infection by integrated bioinformatical analysis and initial clinical validation
研究种属: Human
样品类型: Plasma - 文献: Plasma lipocalin-2/NGAL is stable over 12 weeks and is not modulated by exercise or dieting
研究种属: Human
样品类型: Plasma - 文献: Gingival crevicular fluid lipocalin-2 and semaphorin3A in stage III periodontitis: Non-surgical periodontal treatment effects
研究种属: Human
样品类型: gingival crevicular fluid (GCF) - 文献: Biomarkers of Uremic Cardiotoxicity
研究种属: Human
样品类型: serum - 文献: Serum neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for predicting high dose methotrexate associated acute kidney injury in children with acute lymphoblastic leukemia
研究种属: Human
样品类型: Serum - 文献: Urinary NGAL, IGFBP-7, and TIMP-2: novel biomarkers to predict contrast medium-induced acute kidney injury in children
研究种属: Human
样品类型: Serial urinary samples - 文献: Effects of pneumoperitoneum on kidney injury biomarkers: A randomized clinical trial
研究种属: Human
样品类型: Plasma - 文献: Renal Stem Cells, Renal Resistive Index, and Neutrophil Gelatinase Associated Lipocalin Changes After Revascularization in Patients With Renovascular Hypertension and Ischemic Nephropathy
研究种属: Human
样品类型: serum - 文献: The role of serum and urinary biomarkers in the diagnosis of early diabetic nephropathy in patients with type 2 diabetes
研究种属: Human
样品类型: urinary,serum - 文献: Association of plasma neutrophil gelatinase-associated lipocalin and thoracic aorta calcification in maintenance hemodialysis patients with and without diabetes
样品类型: plasma - 文献: Assessment of acute aerobic exercise in the morning versus evening on asprosin, spexin, lipocalin-2, and insulin level in overweight/obese versus normal weight adult men
研究种属: Human
样品类型: Serum - 文献: Association of plasma neutrophil gelatinase-associated lipocalin with parameters of CKD–MBD in maintenance hemodialysis patients
研究种属: Human
样品类型: plasma - 文献: Evaluation of early biomarkers of renal dysfunction in diabetic patients.
研究种属: Human
样品类型: Urinary - 文献: Perinatal lipocalin-2 profile at the extremes of fetal growth
研究种属: Human
样品类型: milk,serum - 文献: Prognostic utility of neutrophil gelatinase associated lipocalin in cardiac ICU: A prospective study
研究种属: Human
样品类型: serum,urinary - 文献: Impact of colloids or crystalloids in renal function assessed by NGAL and KIM-1 after hysterectomy: randomized controlled trial
研究种属: Human
样品类型: Urine - 文献: Impact of acute versus repetitive moderate intensity endurance exercise on kidney injury markers
研究种属: Human
样品类型: urine - 文献: Impact of acute versus prolonged exercise and dehydration on kidney function and injury
研究种属: Human
样品类型: Urine - 文献: Assessing urinary levels of IL-18, NGAL and albumin creatinine ratio in patients with diabetic nephropathy
研究种属: Human
样品类型: Urine
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实验操作
1. 对应板孔中加入50μL标准品工作液或样本后,立即每孔加入50μL生物素化抗体工作液,37°C孵育90分钟 | |
2. 弃掉板内液体,洗板3次 | |
3. 每孔加入100μL HRP酶结合物工作液,37°C孵育30分钟,弃掉板内液体,洗板5次 | |
4. 每孔加入90μL底物溶液,37°C孵育15分钟左右 | |
5. 每孔加入50μL终止液 | |
6. 立即在450nm波长下读数,处理数据 |
客户发表文献
- 文献: Identification of key candidate biomarkers for severe influenza infection by integrated bioinformatical analysis and initial clinical validation
样品类型: Plasma - 文献: Plasma lipocalin-2/NGAL is stable over 12 weeks and is not modulated by exercise or dieting
样品类型: Plasma - 文献: Gingival crevicular fluid lipocalin-2 and semaphorin3A in stage III periodontitis: Non-surgical periodontal treatment effects
样品类型: gingival crevicular fluid (GCF) - 文献: Biomarkers of Uremic Cardiotoxicity
样品类型: serum - 文献: Serum neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for predicting high dose methotrexate associated acute kidney injury in children with acute lymphoblastic leukemia
样品类型: Serum - 文献: Urinary NGAL, IGFBP-7, and TIMP-2: novel biomarkers to predict contrast medium-induced acute kidney injury in children
样品类型: Serial urinary samples - 文献: Effects of pneumoperitoneum on kidney injury biomarkers: A randomized clinical trial
样品类型: Plasma - 文献: Renal Stem Cells, Renal Resistive Index, and Neutrophil Gelatinase Associated Lipocalin Changes After Revascularization in Patients With Renovascular Hypertension and Ischemic Nephropathy
样品类型: serum - 文献: The role of serum and urinary biomarkers in the diagnosis of early diabetic nephropathy in patients with type 2 diabetes
样品类型: urinary,serum - 文献: Association of plasma neutrophil gelatinase-associated lipocalin and thoracic aorta calcification in maintenance hemodialysis patients with and without diabetes
样品类型: plasma - 文献: Assessment of acute aerobic exercise in the morning versus evening on asprosin, spexin, lipocalin-2, and insulin level in overweight/obese versus normal weight adult men
样品类型: Serum - 文献: Association of plasma neutrophil gelatinase-associated lipocalin with parameters of CKD–MBD in maintenance hemodialysis patients
样品类型: plasma - 文献: Evaluation of early biomarkers of renal dysfunction in diabetic patients.
样品类型: Urinary - 文献: Perinatal lipocalin-2 profile at the extremes of fetal growth
样品类型: milk,serum - 文献: Prognostic utility of neutrophil gelatinase associated lipocalin in cardiac ICU: A prospective study
样品类型: serum,urinary - 文献: Impact of colloids or crystalloids in renal function assessed by NGAL and KIM-1 after hysterectomy: randomized controlled trial
样品类型: Urine - 文献: Impact of acute versus repetitive moderate intensity endurance exercise on kidney injury markers
样品类型: urine - 文献: Impact of acute versus prolonged exercise and dehydration on kidney function and injury
样品类型: Urine - 文献: Assessing urinary levels of IL-18, NGAL and albumin creatinine ratio in patients with diabetic nephropathy
样品类型: Urine