QuicKey Human VEGF-A(Vascular Endothelial Growth Factor A) ELISA Kit
QuicKey-人血管内皮细胞生长因子A(VEGF-A)酶联免疫吸附测定试剂盒
规格: | / 96T / 48T / 96T*5 |
价格: | / ¥2600 / ¥1800 / ¥11700 |
货号:E-TSEL-H0026
货期:3个工作日+顺丰周期
反应性: Human
检测范围: 0.04~10 ng/mL
灵敏度: 0.03 ng/mL
产品信息
QuicKey系列
与传统ELISA试剂盒相比,在实验时间节省至少1小时的同时,获得更加灵敏和精确的实验结果。Elabscience 自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。
基础参数
反应类型 | 双抗体夹心法ELISA |
规格 | 96T/48T |
反应时间 | 2.5 h |
反应性 | 人 |
检测方法 | 比色法 |
检测范围 | 0.04-10 ng/mL |
灵敏度 | 0.03 ng/mL |
上样体积 | 50 μL/孔 |
样本类型 | 血清,血浆 |
特异性 | 可检测样本中的人 VEGF-A , 且与其它类似物无明显交叉反应。 |
重复性 | 板内,板间变异系数均<10%。 |
用途 | 该试剂盒用于体外定量检测人 血清,血浆中VEGF-A浓度。其它相关生物液体请咨询技术支持。 |
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗人 VEGF-A抗体包被于酶标板上,实验时样品(或标准品)及生物素化的抗人 VEGF-A抗体同时加入酶标板中,样本(或标准品)中的人 VEGF-A会与包被抗体结合,同时抗人 VEGF-A抗体与结合在包被抗体上的人 VEGF-A结合,游离的成分被洗去。再加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,VEGF-A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中VEGF-A的浓度。
试剂盒组分及保存
未拆封的试剂盒可在2-8℃ 保存6个月;试剂盒组分开封后请按照下表中的条件分别保存。
中文名称 | 规格 | 开封后保存条件 |
---|---|---|
ELISA 酶标板 |
96T:8 孔×12 条 48T:8 孔×6 条 |
|
冻干标准品 |
96T:2 支 48T:1 支 |
2-8℃,复溶后当天使用 |
标准品&样品稀释液 | 1瓶 20mL | 2-8℃ |
生物素化抗体工作液 | 1瓶 6mL | |
酶结合物稀释液 | 1瓶 14mL | |
浓缩洗涤液 | 1瓶 30mL | |
浓缩HRP酶结合物(100×) |
96T:1支 120μL 48T:1支 60μL |
2-8℃ (避光) |
底物溶液(TMB) | 1瓶 10mL | |
反应终止液 | 1瓶 10mL | 2-8℃ |
封板覆膜 | 5张 | |
产品说明书 | 1 份 | |
质检报告 | 1 份 |
说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。试剂体积以实际发货版说明书为准。 相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。
试验所需自备物品
1. 酶标仪(450nm波长滤光片)
2. 高精度移液器,EP管及一次性吸头:0.5-10μL, 2-20μL, 20-200μL, 200-1000μL
3. 37℃恒温箱,双蒸水或去离子水
4. 吸水纸
技术数据
样本测值参考
标曲示例
以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线 。
浓度:ng/mL | 10 | 3.33 | 1.11 | 0.37 | 0.12 | 0.04 | 0 |
OD | 2.243 | 1.336 | 0.602 | 0.261 | 0.135 | 0.091 | 0.076 |
校正OD | 2.167 | 1.26 | 0.525 | 0.185 | 0.059 | 0.015 | - |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
批内变异系数 | 批间变异系数 | |||||
样本 | 1 | 2 | 3 | 1 | 2 | 3 |
数量 | 20 | 20 | 20 | 20 | 20 | 20 |
平均值(ng/mL) | 0.48 | 1.13 | 4.28 | 0.42 | 1.19 | 4.51 |
标准差 | 0.03 | 0.05 | 0.21 | 0.02 | 0.06 | 0.22 |
变异系数 (%) | 6.25 | 4.42 | 4.91 | 4.76 | 5.04 | 4.88 |
回收率
分别往不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率 。
样本类型 | 回收率范围 (%) | 平均回收率 (%) |
血清(n=8) | 86-97 | 91 |
血浆(EDTA)(n=8) | 82-96 | 90 |
线性
分别往4个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将4个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率 。
血清(n=4) | 血浆(EDTA) (n=4) | ||
1:2 | 回收率范围(%) | 97-110 | 98-104 |
平均回收率(%) | 103 | 101 | |
1:4 | 回收率范围(%) | 97-110 | 84-94 |
平均回收率(%) | 104 | 90 | |
1:8 | 回收率范围(%) | 88-101 | 97-108 |
平均回收率(%) | 93 | 105 | |
1:16 | 回收率范围(%) | 86-95 | 101-108 |
平均回收率(%) | 92 | 104 |
文献引用
- 文献: Sequential oxygen supply system promotes peripheral nerve regeneration by enhancing Schwann cells survival and angiogenesis
研究种属: Human
样品类型: HUVEC - 文献: Regulation of macrophage subtype via injectable micro/nano-structured porous microsphere for reprogramming osteoimmune microenvironment
研究种属: Human
样品类型: HUVECs - 文献: Targeted delivery of fat extract by platelet membrane-cloaked nanocarriers for the treatment of ischemic stroke
样品类型: PLGA-FE - 文献: HIF1α lactylation enhances KIAA1199 transcription to promote angiogenesis and vasculogenic mimicry in prostate cancer
研究种属: Human
样品类型: PC-3 cells,DU145 cells - 文献: A novel mechanism for A-to-I RNA-edited AZIN1 in promoting tumor angiogenesis in colorectal cancer
研究种属: Human
样品类型: HCT-116 cell,HT-29 cell - 文献: Tetrahedral framework nucleic acids promote diabetic wound healing via the Wnt signalling pathway
研究种属: Human
样品类型: HDFs - 文献: Tumor-Contacted Neutrophils Promote Metastasis by a CD90-TIMP-1 Juxtacrine–Paracrine Loop
研究种属: Human
样品类型: tumor-infiltrating neutrophils (TINs),neutrophils - 文献: Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner
研究种属: Human
样品类型: Cell culture supernatant - 文献: Early Osteogenic Marker Expression in hMSCs Cultured onto Acid Etching-Derived Micro- and Nanotopography 3D-Printed Titanium Surfaces
研究种属: Human
样品类型: hBM-MSCs - 文献: Single-Cell Transcriptomics Reveals the Complexity of the Tumor Microenvironment of Treatment-Naive Osteosarcoma
研究种属: Human
样品类型: MG63 cells,U-2 OS cells,Saos-2 cells, 143B cells,K2M2 c - 文献: LncRNA MALAT1 Promotes Tumor Angiogenesis by Regulating MicroRNA-150-5p/VEGFA Signaling in Osteosarcoma: In-Vitro and In-Vivo Analyses
研究种属: Human
样品类型: SaOS2 cell,MG63 cell - 文献: Sesamin inhibits hypoxia-stimulated angiogenesis via NF-κB p65/HIF-1α/VEGFA signaling pathway in human colorectal cancer
研究种属: Human
样品类型: HCT116 cell,SW480 cell - 文献: Self-Assembled Nano-Peptide Hydrogels with Human Umbilical Cord Mesenchymal Stem Cell Spheroids Accelerate Diabetic Skin Wound Healing by Inhibiting Inflammation and Promoting Angiogenesis
研究种属: Human
样品类型: hUC-MSCs - 文献: Efficient Delivery of VEGF-A mRNA for Promoting Diabetic Wound Healing via Ionizable Lipid Nanoparticles
研究种属: Human
样品类型: Human Umbilical Vein Endothelial Cells (HUVECs) - 文献: Homo and Heterotypic Cellular Cross-Talk in Epithelial Ovarian Cancer Impart Pro-Tumorigenic Properties through Differential Activation of the Notch3 Pathway
研究种属: Human
样品类型: SNFT cell - 文献: A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility
研究种属: Human
样品类型: Cell culture supernatant - 文献: Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder
研究种属: Human
样品类型: Human umbilical endothelial cells (HUVEC) - 文献: An effective VEGF-siRNA delivery via folic acid decorated and pegylated silica nanoparticles
研究种属: Human
样品类型: MDA-MD-231 cell,HeLa cell - 文献: GCN5 participates in KLF4-VEGFA feedback to promote endometrial angiogenesis
研究种属: Human
样品类型: HEMECs - 文献: Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction
研究种属: Human
样品类型: Normal human dermal fibroblasts (NHDF) - 文献: MiR-195-5p and miR-205-5p in extracellular vesicles isolated from diabetic foot ulcer wound fluid decrease angiogenesis by inhibiting VEGFA expression
研究种属: Human
样品类型: HUVEC - 文献: Tocotrienol-Rich Vitamin E (Tocovid) Improved Nerve Conduction Velocity in Type 2 Diabetes Mellitus Patients in a Phase II Double-Blind, Randomized Controlled Clinical Trial
研究种属: Human
样品类型: serum - 文献: A Phase IIb Randomized Controlled Trial Investigating the Effects of Tocotrienol-Rich Vitamin E on Diabetic Kidney Disease
研究种属: Human
样品类型: Serum - 文献: Aconiti lateralis radix praeparata total alkaloids exert anti-RA effects by regulating NF-κB and JAK/STAT signaling pathways and promoting apoptosis.
研究种属: Human
样品类型: MH7A cell - 文献: Melatonin stimulates VEGF expression in human granulosa-lutein cells: A potential mechanism for the pathogenesis of ovarian hyperstimulation syndrome
研究种属: Human
样品类型: Follicular Fluid - 文献: Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors
研究种属: Human
样品类型: Cell culture supernatant - 文献: Chrysin Inhibits Melanoma Tumor Metastasis via Interfering with the FOXM1/β-Catenin Signaling
研究种属: Human
样品类型: Cell culture medium - 文献: Adult human periodontal ligament-derived stem cells delay retinal degeneration and maintain retinal function in RCS rats
研究种属: Human
样品类型: human periodontal ligament-derived stem cells (hPDLSCs) - 文献: NLRC5 promotes cell proliferation via regulating the AKT/VEGF-A signaling pathway in hepatocellular carcinoma
研究种属: Human
样品类型: Cell culture supernatant - 文献: Comparison of Serum VEGF, IGF-1, and HIF-1α Levels in Children with Autism Spectrum Disorder and Healthy Controls
研究种属: Human
样品类型: Serum - 文献: The expression and diagnostic value of serum levels of EphA2 and VEGF-A in patients with colorectal cancer
研究种属: Human
样品类型: Serum - 文献: Anti-Angiogenic Activity Of Bevacizumab-Bearing Dexamethasone-Loaded PLGA Nanoparticles For Potential Intravitreal Applications
研究种属: Human
样品类型: HUVECs cells - 文献: LncRNA SPRY4-IT1 facilitates cell proliferation and angiogenesis of glioma via the miR-101-3p/EZH2/VEGFA signaling axis
研究种属: Human
样品类型: U87 cells,SNB19 cells,U118 cells - 文献: Effect of Diosmin Administration in Patients with Chronic Venous Disorders on Selected Factors Affecting Angiogenesis
研究种属: Human
样品类型: serum - 文献: REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells
研究种属: Human
样品类型: Cell lysate - 文献: Associations among serum VEGF and CGRP levels with the peripheral vascular blood flow of the skin of the hands in women with Fibromyalgia
研究种属: Human
样品类型: Serum - 文献: The relationship of plasma miR-503 and coronary collateral circulation in patients with coronary artery disease
研究种属: Human
样品类型: Plasma - 文献: Association of rs699947 (−2578 C/A) and rs2010963 (−634 G/C) Single Nucleotide Polymorphisms of the VEGF Gene, VEGF-A and Leptin Serum Level, and Cardiovascular Risk in Patients with Excess Body Mass: A Case–Control Study
研究种属: Human
样品类型: Serum - 文献: Efficient Delivery of VEGFA mRNA for Promoting Wound Healing via Ionizable Lipid Nanoparticles
研究种属: Human
样品类型: Human Umbilical Vein Endothelial Cells (HUVECs) - 文献: Context dependent role of p53 during the interaction of hepatocellular carcinoma and endothelial cells
研究种属: Human
样品类型: HepG2 cell - 文献: Melatonin regulates tumor angiogenesis via miR-424-5p/VEGFA signaling pathway in osteosarcoma
研究种属: Human
样品类型: Cell lysate - 文献: Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways
研究种属: Human
样品类型: Serum,Cell culture supernatant - 文献: Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro
研究种属: Human
样品类型: Wound drainage fluid - 文献: Evaluation of serum vascular endothelial growth factor level and findings of nailfold capillaroscopy by dermatoscope in the differential diagnosis of palmoplantar psoriasis and palmoplantar eczema
研究种属: Human
样品类型: serum - 文献: Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis
研究种属: Human
样品类型: Cell culture supernatant - 文献: The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of MSCs (human and rat) and HUVECs co-culture models
研究种属: Human
样品类型: VEGF solution after immobilization - 文献: Comparison of VEGF‐A Values between Pregnant Women with COVID‐19 and Healthy Pregnancies and Its Association with Composite Adverse Outcomes
研究种属: Human
样品类型: Whole Blood - 文献: The Prognostic Value of Circulating VEGF-A Level in Patients With Hepatocellular Cancer:
研究种属: Human
样品类型: plasma - 文献: Reduced Plasma miR-146a Is a Predictor of Poor Coronary Collateral Circulation in Patients with Coronary Artery Disease
研究种属: Human
样品类型: Plasma - 文献: Increased serum concentration of netrin-1 after intravitreal bevacizumab injection: is it a compensatory mechanism to counteract drug side effects?
研究种属: Human
样品类型: Serum - 文献: Sinensetin suppresses angiogenesis in liver cancer by targeting the VEGF/VEGFR2/AKT signaling pathway
研究种属: Human
样品类型: HepG2/C3A cell - 文献: Serum VEGF-A and VEGFR-1 levels in patients with adult immunoglobulin A vasculitis
研究种属: Human
样品类型: Serum - 文献: Thicker carotid intima-media thickness and increased plasma VEGF levels suffered by post-acute thrombotic stroke patients
研究种属: Human
样品类型: Plasma - 文献: Changes of biochemical biomarkers in the serum of children with convulsion status epilepticus: a prospective study
研究种属: Human
样品类型: serum - 文献: Maternal Serum VEGF Predicts Abnormally Invasive Placenta Better than NT-proBNP: a Multicenter Case-Control Study
研究种属: Human
样品类型: Serum - 文献: The rhenium(I)-diselenoether anticancer drug targets ROS, TGF-β1, VEGF-A, and IGF-1 in an in vitro experimental model of triple-negative breast cancers
研究种属: Human
样品类型: Cell culture medium - 文献: Relationship between elevated microRNAs and growth factors levels in the vitreous of patients with proliferative diabetic retinopathy
研究种属: Human
样品类型: vitreous sample - 文献: The relationship of plasma decoy receptor 3 and coronary collateral circulation in patients with coronary artery disease
研究种属: Human
样品类型: Plasma - 文献: polymorphisms with diabetic retinopathy in Pakistani population
研究种属: Human
样品类型: Serum - 文献: The Antiangiogenesis Effect of Pirfenidone in Wound Healing In Vitro
研究种属: Human
样品类型: Cell culture supernatant - 文献: Vascular endothelial growth factor and pulmonary hypertension in children with beta thalassemia major
研究种属: Human
样品类型: Serum - 文献: Angiogenic markers predict kidney injury and obstetric complications in women with preeclampsia and pregnancy-related acute kidney injury
研究种属: Human
样品类型: Serum - 文献: Autophagy: a new mechanism for regulating VEGF and PEDF expression in retinal pigment epithelium cells
研究种属: Human
样品类型: ARPE-19 cells - 文献: Expression and predictive value of HIF‑1α and VEGF in patients with burns following treatment
研究种属: Human
样品类型: Serum - 文献: Clinical outcome and prognostic analysis of meningeal carcinomatosis treated by intrathecal chemotherapy
研究种属: Human
样品类型: cell - 文献: Evaluation of the growth factors VEGF-a and VEGF-B in the vitreous and serum of patients with macular and retinal vascular diseases
研究种属: Human
样品类型: Serum - 文献: Comparison between Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor Levels in Rhegmatogenous Retinal Detachment
研究种属: Human
样品类型: serum - 文献: Apelin-13: A Promising Biomarker for Age-Related Macular Degeneration?
研究种属: Human
样品类型: serum - 文献: Comparison of the Cytokine Profile in Mesenchymal Stem Cells from Human Adipose, Umbilical Cord, and Placental Tissues
研究种属: Human
样品类型: mesenchymal stem cells (MSCs) - 文献: Vitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy
研究种属: Human
样品类型: vitreous,serum - 文献: The effects of vitamin E on non-proliferative diabetic retinopathy in type 2 diabetes mellitus: Are they sustainable with 12 months of therapy:
研究种属: Human
样品类型: serum - 文献: Serum levels of vasoactive factors in HIV-infected pre-eclamptic women on HAART
研究种属: Human
样品类型: serum - 文献: Serum Angiogenic and Anti-angiogenic Markers in Pregnant Women with Placenta Percreta
研究种属: Human
样品类型: Serum - 文献: Osmolyte taurine induction in UVA exposed human retinal pigment epithelial cells
研究种属: Human
样品类型: Cells
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实验操作
1. 对应板孔中加入50μL标准品工作液或样本后,立即每孔加入50μL生物素化抗体工作液,37°C孵育90分钟 | |
2. 弃掉板内液体,洗板3次 | |
3. 每孔加入100μL HRP酶结合物工作液,37°C孵育30分钟,弃掉板内液体,洗板5次 | |
4. 每孔加入90μL底物溶液,37°C孵育15分钟左右 | |
5. 每孔加入50μL终止液 | |
6. 立即在450nm波长下读数,处理数据 |
客户发表文献
- 文献: Sequential oxygen supply system promotes peripheral nerve regeneration by enhancing Schwann cells survival and angiogenesis
样品类型: HUVEC - 文献: Regulation of macrophage subtype via injectable micro/nano-structured porous microsphere for reprogramming osteoimmune microenvironment
样品类型: HUVECs - 文献: Targeted delivery of fat extract by platelet membrane-cloaked nanocarriers for the treatment of ischemic stroke
样品类型: PLGA-FE - 文献: HIF1α lactylation enhances KIAA1199 transcription to promote angiogenesis and vasculogenic mimicry in prostate cancer
样品类型: PC-3 cells,DU145 cells - 文献: A novel mechanism for A-to-I RNA-edited AZIN1 in promoting tumor angiogenesis in colorectal cancer
样品类型: HCT-116 cell,HT-29 cell - 文献: Tetrahedral framework nucleic acids promote diabetic wound healing via the Wnt signalling pathway
样品类型: HDFs - 文献: Tumor-Contacted Neutrophils Promote Metastasis by a CD90-TIMP-1 Juxtacrine–Paracrine Loop
样品类型: tumor-infiltrating neutrophils (TINs),neutrophils - 文献: Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner
样品类型: Cell culture supernatant - 文献: Early Osteogenic Marker Expression in hMSCs Cultured onto Acid Etching-Derived Micro- and Nanotopography 3D-Printed Titanium Surfaces
样品类型: hBM-MSCs - 文献: Single-Cell Transcriptomics Reveals the Complexity of the Tumor Microenvironment of Treatment-Naive Osteosarcoma
样品类型: MG63 cells,U-2 OS cells,Saos-2 cells, 143B cells,K2M2 c - 文献: LncRNA MALAT1 Promotes Tumor Angiogenesis by Regulating MicroRNA-150-5p/VEGFA Signaling in Osteosarcoma: In-Vitro and In-Vivo Analyses
样品类型: SaOS2 cell,MG63 cell - 文献: Sesamin inhibits hypoxia-stimulated angiogenesis via NF-κB p65/HIF-1α/VEGFA signaling pathway in human colorectal cancer
样品类型: HCT116 cell,SW480 cell - 文献: Self-Assembled Nano-Peptide Hydrogels with Human Umbilical Cord Mesenchymal Stem Cell Spheroids Accelerate Diabetic Skin Wound Healing by Inhibiting Inflammation and Promoting Angiogenesis
样品类型: hUC-MSCs - 文献: Efficient Delivery of VEGF-A mRNA for Promoting Diabetic Wound Healing via Ionizable Lipid Nanoparticles
样品类型: Human Umbilical Vein Endothelial Cells (HUVECs) - 文献: Homo and Heterotypic Cellular Cross-Talk in Epithelial Ovarian Cancer Impart Pro-Tumorigenic Properties through Differential Activation of the Notch3 Pathway
样品类型: SNFT cell - 文献: A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility
样品类型: Cell culture supernatant - 文献: Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder
样品类型: Human umbilical endothelial cells (HUVEC) - 文献: An effective VEGF-siRNA delivery via folic acid decorated and pegylated silica nanoparticles
样品类型: MDA-MD-231 cell,HeLa cell - 文献: GCN5 participates in KLF4-VEGFA feedback to promote endometrial angiogenesis
样品类型: HEMECs - 文献: Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction
样品类型: Normal human dermal fibroblasts (NHDF) - 文献: MiR-195-5p and miR-205-5p in extracellular vesicles isolated from diabetic foot ulcer wound fluid decrease angiogenesis by inhibiting VEGFA expression
样品类型: HUVEC - 文献: Tocotrienol-Rich Vitamin E (Tocovid) Improved Nerve Conduction Velocity in Type 2 Diabetes Mellitus Patients in a Phase II Double-Blind, Randomized Controlled Clinical Trial
样品类型: serum - 文献: A Phase IIb Randomized Controlled Trial Investigating the Effects of Tocotrienol-Rich Vitamin E on Diabetic Kidney Disease
样品类型: Serum - 文献: Aconiti lateralis radix praeparata total alkaloids exert anti-RA effects by regulating NF-κB and JAK/STAT signaling pathways and promoting apoptosis.
样品类型: MH7A cell - 文献: Melatonin stimulates VEGF expression in human granulosa-lutein cells: A potential mechanism for the pathogenesis of ovarian hyperstimulation syndrome
样品类型: Follicular Fluid - 文献: Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors
样品类型: Cell culture supernatant - 文献: Chrysin Inhibits Melanoma Tumor Metastasis via Interfering with the FOXM1/β-Catenin Signaling
样品类型: Cell culture medium - 文献: Adult human periodontal ligament-derived stem cells delay retinal degeneration and maintain retinal function in RCS rats
样品类型: human periodontal ligament-derived stem cells (hPDLSCs) - 文献: NLRC5 promotes cell proliferation via regulating the AKT/VEGF-A signaling pathway in hepatocellular carcinoma
样品类型: Cell culture supernatant - 文献: Comparison of Serum VEGF, IGF-1, and HIF-1α Levels in Children with Autism Spectrum Disorder and Healthy Controls
样品类型: Serum - 文献: The expression and diagnostic value of serum levels of EphA2 and VEGF-A in patients with colorectal cancer
样品类型: Serum - 文献: Anti-Angiogenic Activity Of Bevacizumab-Bearing Dexamethasone-Loaded PLGA Nanoparticles For Potential Intravitreal Applications
样品类型: HUVECs cells - 文献: LncRNA SPRY4-IT1 facilitates cell proliferation and angiogenesis of glioma via the miR-101-3p/EZH2/VEGFA signaling axis
样品类型: U87 cells,SNB19 cells,U118 cells - 文献: Effect of Diosmin Administration in Patients with Chronic Venous Disorders on Selected Factors Affecting Angiogenesis
样品类型: serum - 文献: REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells
样品类型: Cell lysate - 文献: Associations among serum VEGF and CGRP levels with the peripheral vascular blood flow of the skin of the hands in women with Fibromyalgia
样品类型: Serum - 文献: The relationship of plasma miR-503 and coronary collateral circulation in patients with coronary artery disease
样品类型: Plasma - 文献: Association of rs699947 (−2578 C/A) and rs2010963 (−634 G/C) Single Nucleotide Polymorphisms of the VEGF Gene, VEGF-A and Leptin Serum Level, and Cardiovascular Risk in Patients with Excess Body Mass: A Case–Control Study
样品类型: Serum - 文献: Efficient Delivery of VEGFA mRNA for Promoting Wound Healing via Ionizable Lipid Nanoparticles
样品类型: Human Umbilical Vein Endothelial Cells (HUVECs) - 文献: Context dependent role of p53 during the interaction of hepatocellular carcinoma and endothelial cells
样品类型: HepG2 cell - 文献: Melatonin regulates tumor angiogenesis via miR-424-5p/VEGFA signaling pathway in osteosarcoma
样品类型: Cell lysate - 文献: Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways
样品类型: Serum,Cell culture supernatant - 文献: Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro
样品类型: Wound drainage fluid - 文献: Evaluation of serum vascular endothelial growth factor level and findings of nailfold capillaroscopy by dermatoscope in the differential diagnosis of palmoplantar psoriasis and palmoplantar eczema
样品类型: serum - 文献: Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis
样品类型: Cell culture supernatant - 文献: The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of MSCs (human and rat) and HUVECs co-culture models
样品类型: VEGF solution after immobilization - 文献: Comparison of VEGF‐A Values between Pregnant Women with COVID‐19 and Healthy Pregnancies and Its Association with Composite Adverse Outcomes
样品类型: Whole Blood - 文献: The Prognostic Value of Circulating VEGF-A Level in Patients With Hepatocellular Cancer:
样品类型: plasma - 文献: Reduced Plasma miR-146a Is a Predictor of Poor Coronary Collateral Circulation in Patients with Coronary Artery Disease
样品类型: Plasma - 文献: Increased serum concentration of netrin-1 after intravitreal bevacizumab injection: is it a compensatory mechanism to counteract drug side effects?
样品类型: Serum - 文献: Sinensetin suppresses angiogenesis in liver cancer by targeting the VEGF/VEGFR2/AKT signaling pathway
样品类型: HepG2/C3A cell - 文献: Serum VEGF-A and VEGFR-1 levels in patients with adult immunoglobulin A vasculitis
样品类型: Serum - 文献: Thicker carotid intima-media thickness and increased plasma VEGF levels suffered by post-acute thrombotic stroke patients
样品类型: Plasma - 文献: Changes of biochemical biomarkers in the serum of children with convulsion status epilepticus: a prospective study
样品类型: serum - 文献: Maternal Serum VEGF Predicts Abnormally Invasive Placenta Better than NT-proBNP: a Multicenter Case-Control Study
样品类型: Serum - 文献: The rhenium(I)-diselenoether anticancer drug targets ROS, TGF-β1, VEGF-A, and IGF-1 in an in vitro experimental model of triple-negative breast cancers
样品类型: Cell culture medium - 文献: Relationship between elevated microRNAs and growth factors levels in the vitreous of patients with proliferative diabetic retinopathy
样品类型: vitreous sample - 文献: The relationship of plasma decoy receptor 3 and coronary collateral circulation in patients with coronary artery disease
样品类型: Plasma - 文献: polymorphisms with diabetic retinopathy in Pakistani population
样品类型: Serum - 文献: The Antiangiogenesis Effect of Pirfenidone in Wound Healing In Vitro
样品类型: Cell culture supernatant - 文献: Vascular endothelial growth factor and pulmonary hypertension in children with beta thalassemia major
样品类型: Serum - 文献: Angiogenic markers predict kidney injury and obstetric complications in women with preeclampsia and pregnancy-related acute kidney injury
样品类型: Serum - 文献: Autophagy: a new mechanism for regulating VEGF and PEDF expression in retinal pigment epithelium cells
样品类型: ARPE-19 cells - 文献: Expression and predictive value of HIF‑1α and VEGF in patients with burns following treatment
样品类型: Serum - 文献: Clinical outcome and prognostic analysis of meningeal carcinomatosis treated by intrathecal chemotherapy
样品类型: cell - 文献: Evaluation of the growth factors VEGF-a and VEGF-B in the vitreous and serum of patients with macular and retinal vascular diseases
样品类型: Serum - 文献: Comparison between Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor Levels in Rhegmatogenous Retinal Detachment
样品类型: serum - 文献: Apelin-13: A Promising Biomarker for Age-Related Macular Degeneration?
样品类型: serum - 文献: Comparison of the Cytokine Profile in Mesenchymal Stem Cells from Human Adipose, Umbilical Cord, and Placental Tissues
样品类型: mesenchymal stem cells (MSCs) - 文献: Vitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy
样品类型: vitreous,serum - 文献: The effects of vitamin E on non-proliferative diabetic retinopathy in type 2 diabetes mellitus: Are they sustainable with 12 months of therapy:
样品类型: serum - 文献: Serum levels of vasoactive factors in HIV-infected pre-eclamptic women on HAART
样品类型: serum - 文献: Serum Angiogenic and Anti-angiogenic Markers in Pregnant Women with Placenta Percreta
样品类型: Serum - 文献: Osmolyte taurine induction in UVA exposed human retinal pigment epithelial cells
样品类型: Cells