QuicKey Pro Human IL-6(Interleukin 6) ELISA Kit
QuicKey Pro-人白介素6(IL-6)酶联免疫吸附测定试剂盒
规格: | / 96T / 48T / 96T*5 |
价格: | / ¥2400 / ¥1650 / ¥10800 |
货号:E-OSEL-H0001
反应性: 人
检测范围: 3.13~200 pg/mL
灵敏度: 1.02 pg/mL
QuicKey Pro系列
与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。
用途
该试剂盒用于体外定量检测人血清、血浆或其他相关生物液体中IL-6浓度。
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗人 IL-6抗体包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗IL-6检测抗体,样本(或标准品中)的IL-6与包被抗体IL-6及HRP标记的抗IL-6抗体结合,形成包被抗体-IL-6-HRP标记的抗IL-6抗体的复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-6浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-6的浓度。
反应类型 | 双抗体夹心法 |
规格 | 96T |
反应时间 | 1.5h |
反应性 | 人 |
检测方法 | Colormetric |
检测范围 | 3.13-200 pg/mL |
灵敏度 | 1.02 pg/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆或其他相关生物液体 |
特异性
可检测样本中的人IL-6,且与其它相关蛋白无明显交叉反应。
重复性
板内,板间变异系数均<10%。
典型数据
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) |
9.10 | 22.29 | 90.92 | 9.12 | 21.98 | 90.28 |
Standard
deviation |
0.57 | 1.13 | 3.32 | 0.53 | 1.31 | 3.25 |
C V (%) | 6.24 | 5.06 | 3.65 | 5.84 | 5.98 | 3.60 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum (n=8) | 89-102 | 96 |
EDTA plasma (n=8) | 92-105 | 97 |
线性
分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。
Serum (n=5) | EDTA plasma (n=5) | ||
---|---|---|---|
1:2 | Range (%) | 94-107 | 85-99 |
Average (%) | 102 | 91 | |
1:4 | Range (%) | 97-111 | 85-95 |
Average (%) | 102 | 90 | |
1:8 | Range (%) | 95-108 | 86-98 |
Average (%) | 100 | 93 | |
1:16 | Range (%) | 96-111 | 83-97 |
Average (%) | 102 | 88 |
试剂盒组成及保存
未拆封的试剂盒可在2-8℃保存6个月;试剂盒组分开封后请按照下表中的条件分别保存。
中文名称 | 英文名称 | 规格 | 保存条件 |
---|---|---|---|
ELISA 酶标板 | Micro ELISA Plate (Dismountable) | 96T:8 孔×12 条 48T:8 孔×6 条 24T:8 孔×3 条 96T*5:5 块 96T酶标板 |
2-8℃, 可存放1个月 |
冻干标准品 | Reference Standard | 96T:2 支 48T/24T:1 支 96T*5:10 支 |
2-8℃, 复溶后当天使用 |
浓缩HRP酶结合抗体 (100×) | Concentrated HRP Linked Detection Ab(100×) | 96T:1支 60μL 48T/24T:1支 30μL 96T*5:5 支 60μL |
2-8℃(避光) |
标准品&样品稀释液 | Reference Standard & Sample Diluent | 96T/48T/24T:1瓶 10mL 96T*5:5瓶 10mL |
2-8℃ |
HRP酶结合抗体稀释液 | HRP Linked Ab Diluent | 96T/48T/24T:1瓶 7mL 96T*5:5瓶 7mL |
|
酶结合物稀释液 | Concentrated Wash Buffer(25×) | 96T/48T/24T:1瓶 30mL 96T*5:5瓶 30mL |
|
底物溶液(TMB) | Substrate Reagent | 96T/48T/24T:1瓶 10mL 96T*5:5瓶 10mL |
2-8℃(避光) |
反应终止液 | Stop Solution | 96T/48T/24T:1瓶 10mL 96T*5:5瓶 10mL |
2-8℃ |
封板覆膜 | Plate Sealer | 96T/48T/24T:5张 96T*5:25张 |
|
产品说明书 | Product Description | 1 份 | |
质检报告 | Certificate of Analysis | 1 份 |
说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。
试验所需自备物品
- 1.酶标仪(450nm波长滤光片)
- 2.高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3.37℃恒温箱,双蒸水或去离子水
- 4.吸水纸
操作步骤
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样品类型: Cell culture supernatant(HMECs) - 文献: Mesenchymal Stem Cells (MSCs) Coculture Protects [Ca2+]i Orchestrated Oxidant Mediated Damage in Differentiated Neurons In Vitro
样品类型: Cell culture supernatant - 文献: Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4/NF-κB/STAT3 Activation in Ox-LDL-Treated HUVECs
样品类型: Cell culture supernatant - 文献: CALML6 Controls TAK1 Ubiquitination and Confers Protection against Acute Inflammation
样品类型: Cell culture supernatant - 文献: Potential Therapeutic Activity of Berberine in Thyroid-Associated Ophthalmopathy: Inhibitory Effects on Tissue Remodeling in Orbital Fibroblasts
样品类型: Orbital fibroblasts (OFs) - 文献: Modulatory Effect of Diosmin and Diosmetin on Metalloproteinase Activity and Inflammatory Mediators in Human Skin Fibroblasts Treated with Lipopolysaccharide
样品类型: human fibroblast cell - 文献: Arsenic trioxide improves Treg and Th17 balance by modulating STAT3 in treatment-naïve rheumatoid arthritis patients
样品类型: PBMCs cells - 文献: Altered kynurenine pathway metabolism in patients with ankylosing spondylitis
样品类型: serum - 文献: PPAR-Alpha Agonist Fenofibrate Combined with Octreotide Acetate in the Treatment of Acute Hyperlipidemia Pancreatitis
样品类型: Serum - 文献: Arsenic Trioxide in Synergy with Vitamin D Rescues the Defective VDR-PPAR- Functional Module of Autophagy in Rheumatoid Arthritis
样品类型: rheumatoid arthritis (RA) fibroblast-like synoviocytes - 文献: MiR-942-5p alleviates septic acute kidney injury by targeting FOXO3.
样品类型: Cell culture supernatant - 文献: The association of the quality of sleep with proinflammatory cytokine profile in inflammatory bowel disease patients
样品类型: serum - 文献: Role of CD36 rs1761667 AA genotype in the expression of inflammatory cytokines and Parkinson’s disease progression: A case-control study in a northern Han Chinese population
样品类型: plasma - 文献: Effect of Diosmin Administration in Patients with Chronic Venous Disorders on Selected Factors Affecting Angiogenesis
样品类型: serum - 文献: LncRNA MALAT1 promotes relapse of breast cancer patients with postoperative fever
样品类型: plasma - 文献: Common variants of genes encoding TLR4 and TLR4 pathway members TIRAP and IRAK1 are effective on MCP1, IL6, IL1β, and TNFα levels in type 2 diabetes and insulin resistance
样品类型: serum - 文献: IL-16 regulates macrophage polarization as a target gene of mir-145-3p
样品类型: Cell lysate - 文献: A role for serum cytokines and cell adhesion molecules in the non‑invasive diagnosis of colorectal cancer
样品类型: serum - 文献: Asiatic acid from Potentilla chinensis alleviates non-alcoholic fatty liver by regulating endoplasmic reticulum stress and lipid metabolism
样品类型: HepG2 cells - 文献: TSG attenuates LPC‐induced endothelial cells inflammatory damage through notch signaling inhibition
样品类型: Cell culture supernatant - 文献: Inflammatory Biomarkers Levels in T2DM Emirati Patients with Diabetic Neuropathy
样品类型: serum - 文献: LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203
样品类型: Cell culture supernatant - 文献: Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals
样品类型: Serum - 文献: Oxidative Burst of Circulating Neutrophils Following Traumatic Brain Injury in Human
样品类型: Plasma - 文献: Clinical characteristics and outcomes of COVID-19 patients in Bali, Indonesia
样品类型: serum - 文献: Transcriptome analysis of eutopic endometrial stromal cells in women with adenomyosis by RNA-sequencing
样品类型: A-EuESCs,N-ESCs - 文献: Nintedanib ameliorates oxidized low-density lipoprotein -induced inflammation and cellular senescence in vascular endothelial cells
样品类型: HUVECs - 文献: Dihydroartemisinin targets fibroblast growth factor receptor 1 (FGFR1) to inhibit interleukin 17A (IL-17A)-induced hyperproliferation and inflammation of keratinocytes
样品类型: HaCaT cell - 文献: Circ_0001897 regulates high glucose-induced angiogenesis and inflammation in retinal microvascular endothelial cells through miR-29c-3p/transforming growth factor beta 2 axis
样品类型: Human retinal microvascular endothelial cell (HRECs) - 文献: Carvedilol activates nuclear factor E2-related factor 2/ antioxidant response element pathway to inhibit oxidative stress and apoptosis of retinal pigment epithelial cells induced by high glucose
样品类型: ARPE-19 cell - 文献: Apremilast mitigates interleukin (IL)-13-induced inflammatory response and mucin production in human nasal epithelial cells (hNECs)
样品类型: Human nasal epithelial cells (hNECs) - 文献: The development of an ultra-sensitive electrochemical immunosensor using a PPyr-NHS functionalized disposable ITO sheet for the detection of interleukin 6 in real human serums
样品类型: serum - 文献: DosR antigen Rv1737c induces activation of macrophages dependent on the TLR2 pathway
样品类型: THP-1 cells - 文献: ADSC-conditioned media elicit an ex vivo anti-inflammatory macrophage response
样品类型: Serum,Cell culture medium - 文献: The first cloned echinoderm tumor necrosis factor receptor from Holothuria leucospilota: Molecular characterization and functional analysis
样品类型: Cell culture supernatant - 文献: Comparison of delta-tocotrienol and alpha-tocopherol effects on hepatic steatosis and inflammatory biomarkers in patients with non-alcoholic fatty liver disease: A randomized double-blind active-controlled trial
样品类型: serum - 文献: Long non-coding RNA NEAT1 promotes lipopolysaccharide-induced injury in human tubule epithelial cells by regulating miR-93-5p/TXNIP axis
样品类型: Cell culture supernatant - 文献: Cul4a attenuates LPS-induced acute kidney injury via blocking NF-kB signaling pathway in sepsis
样品类型: HK-2 cell - 文献: Iron Status in Newly Diagnosed β-Thalassemia Major: High Rate of Iron Status due to Erythropoiesis Drive
样品类型: Serum - 文献: Eriodictyol inhibits survival and inflammatory responses and promotes apoptosis in rheumatoid arthritis fibroblast‐like synoviocytes through AKT/FOXO1 signaling
样品类型: rheumatoid arthritis (RA) fibroblast-like synoviocytes - 文献: The Value of Extracellular Cold-Inducible RNA-Binding Protein (eCIRP) in Predicting the Severity and Prognosis of Patients After Cardiac Arrest: A Preliminary Observational Study
样品类型: serum - 文献: Association between pro-inflammatory cytokines in cerebrospinal fluid and headache in patients with aneurysmal subarachnoid hemorrhage
样品类型: cerebrospinal fluid (CSF) - 文献: The Role of Plasma Interleukin-6 Levels on Atherosclerotic Cardiovascular Disease and Cardiovascular Mortality Risk Scores in Javanese Patients with Chronic Kidney Disease
样品类型: plasma - 文献: NLRP6 Induces Lung Injury and Inflammation Early in Brucella and Influenza Coinfection
样品类型: lung tissues - 文献: Nomogram Based on Cytokines for Cardiovascular Diseases in Xinjiang Kazakhs
样品类型: plasma - 文献: Grape Seed Proanthocyanidin Extract Inhibits Human Esophageal Squamous Cancerous Cell Line ECA109 via the NF-κB Signaling Pathway
样品类型: ECA109 cells - 文献: Bradykinin/bradykinin 1 receptor promotes brain microvascular endothelial cell permeability and proinflammatory cytokine release by downregulating Wnt3a
样品类型: serum,hBMECs - 文献: Nec-1 Attenuates Neurotoxicity Induced by Titanium Dioxide Nanomaterials on Sh-Sy5y Cells Through RIP1
样品类型: SH-SY5Y cell - 文献: Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways
样品类型: Cell culture supernatant - 文献: Expression of Nrf2 protein in serum of patients with rheumatoid arthritis: A novel indicator for disease activity and disease prognosis
样品类型: serum - 文献: strain FP10
样品类型: Cell culture supernatant - 文献: Does the TMPRSS6 C > T Polymorphism Modify the Endurance Training Effects on Hematological Parameters?
样品类型: serum - 文献: Impact of Lipophilic Versus Hydrophilic Statins on the Clinical Outcome and Biomarkers of Remodeling in Heart Failure Patients: A Prospective Comparative Randomized Study
样品类型: serum - 文献: Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro
样品类型: Wound drainage fluid - 文献: Diagnostic and Prognostic Value of IL-6 and sTREM-1 in SIRS and Sepsis in Children
样品类型: Serum - 文献: Chronic fatigue and fibromyalgia symptoms are key components of deficit schizophrenia and are strongly associated with activated immune-inflammatory pathways
样品类型: Serum - 文献: Role of non-neuronal cholinergic system in the early stage response of epithelial-mesenchymal transformation related markers in A549 cells induced by coal particles
样品类型: A549 cell - 文献: Tocilizumab attenuates acute lung and kidney injuries and improves survival in a rat model of sepsis via down-regulation of NF-κB/JNK: a possible role of P-glycoprotein
样品类型: Serum - 文献: MicroRNA-218 acts by repressing TNFR1-mediated activation of NF-κB, which is involved in MUC5AC hyper-production and inflammation in smoking-induced bronchiolitis of COPD
样品类型: Serum,Cell culture supernatant - 文献: Interleukin-6, undercarboxylated osteocalcin, and brain-derived neurotrophic factor responses to single and repeated sessions of high-intensity interval exercise
样品类型: plasma - 文献: Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma
样品类型: THP-1 cell - 文献: The liver X receptor agonist T0901317 reduces the inflammation of alveolar epithelial cells induced by polyhexamethylene guanidine through inhibition of the NFκB signaling pathway
样品类型: A549 cell - 文献: Possible causes of atherosclerosis: lncRNA COLCA1 induces oxidative stress in human coronary artery endothelial cells and impairs wound healing
样品类型: HCAECs - 文献: Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF
样品类型: adipose-derived stem cell (ASCs) - 文献: Mammalian Ste20-like kinase 4 inhibits the inflammatory response in Aspergillus fumigatus keratitis
样品类型: Cell lysate - 文献: Inhibition of HMGB1 involved in the protective of salidroside on liver injury in diabetes mice
样品类型: Cell culture supernatant - 文献: IL-38 alleviates the inflammatory response and the degeneration of nucleus pulposus cells via inhibition of the NF-κB signaling pathway in vitro
样品类型: Cell culture supernatant - 文献: Clonorchis sinensis Co-infection Could Affect the Disease State and Treatment Response of HBV Patients
样品类型: Serum,Cell culture supernatant - 文献: Somatization is associated with altered serum levels of vitamin D, serotonin, and brain-derived neurotrophic factor in patients with predominant diarrhea irritable bowel syndrome
样品类型: serum - 文献: Silencing of UCA1 Protects Against MPP+-Induced Cytotoxicity in SK-N-SH Cells via Modulating KCTD20 Expression by Sponging miR-423-5p
样品类型: Cell culture supernatant - 文献: Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits
样品类型: Cell culture supernatant - 文献: The effect of different dietary ratios of arginine, methionine, and lysine on the performance, carcass traits, and immune status of turkeys
样品类型: Plasma - 文献: Evaluation of serum semaphorin 3A and interleukin 6 levels in patients with pseudoexfoliation syndrome
样品类型: serum - 文献: Investigation of DNA damage, oxidative stress, and inflammation in synthetic cannabinoid users:
样品类型: Plasma - 文献: Penehyclidine hydrochloride alleviates lipopolysaccharide‑induced acute respiratory distress syndrome in cells via regulating autophagy‑related pathway
样品类型: Cell culture supernatant - 文献: Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes
样品类型: Serum - 文献: Correlation of Inflammation and Coagulation Markers with the Incidence of Deep Vein Thrombosis in Cancer Patients with High Risk of Thrombosis
样品类型: plasma - 文献: Intermittent hypoxia induces tumor immune escape in murine S180 solid tumors via the upregulation of TGF-β 1 in mice
样品类型: Tissue homogenate - 文献: Functional characterisation of Holothuria leucospilota Fas-associated death domain in the innate immune-related signalling pathways:
样品类型: Cell culture supernatant - 文献: Nigella sativa Oil Mouth Rinse Improves Chemotherapy-Induced Oral Mucositis in Patients with Acute Myeloid Leukemia
样品类型: salivary - 文献: Galectin-3, IL-1A, IL-6, and EGF Levels in Corneal Epithelium of Patients With Recurrent Corneal Erosion Syndrome
样品类型: Tissue homogenate - 文献: A Self-Representation-Based Fuzzy SVM Model for Predicting Vascular Calcification of Hemodialysis Patients
样品类型: serum - 文献: Association of the IRAK4 rs4251545 genetic polymorphism with severity of enterovirus-71 infection in Chinese children
样品类型: plasma - 文献: Evaluation of malnutrition and inflammation after total parathyroidectomy in patients on maintenance dialysis
样品类型: serum - 文献: Effect of Lycium barbarum polysaccharide supplementation in non-alcoholic fatty liver disease patients: study protocol for a randomized controlled trial
样品类型: blood - 文献: Roles and mechanisms of NRG1 in modulating the pathogenesis of NAFLD through ErbB3 signaling in hepatocytes (NRG1 modulates NAFLD through ErbB3 signaling)
样品类型: Cell culture supernatant - 文献: MicroRNA-33-5p inhibits cholesterol efflux in vascular endothelial cells by regulating citrate synthase and ATP-binding cassette transporter A1
样品类型: VEC - 文献: Evaluation of the relationship between pentraxin 3 (PTX3) rs2305619 (281A/G) and rs1840680 (1449A/G) polymorphisms and the clinical course of COVID-19
样品类型: blood - 文献: Analysis of serum cytokine and protective vitamin D levels in severe cases of COVID-19
样品类型: serum - 文献: Leptin induces IL-6 and IL-8 expression through leptin receptor Ob-Rb in human dental pulp fibroblasts
样品类型: Cell culture supernatant - 文献: Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes
样品类型: Cell culture supernatant - 文献: A case of asthma with Behcet’s disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions
样品类型: Serum - 文献: Circ_0043947 contributes to interleukin 1β-induced injury in chondrocytes by sponging miR-671-5p to up-regulate RTN3 expression in osteoarthritis pathology
样品类型: chondrocytes - 文献: C reactive protein and enzymatically modified LDL cooperatively promote dendritic cell-mediated T cell activation
样品类型: Cell culture supernatant - 文献: Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells
样品类型: Cell lysate - 文献: Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line
样品类型: Cell lysate - 文献: Role of resveratrol supplementation in regulation of glucose hemostasis, inflammation and oxidative stress in patients with diabetes mellitus type 2: A randomized, placebo-controlled trial
样品类型: serum - 文献: MicroRNA‑16 regulates lipopolysaccharide‑induced inflammatory factor expression by targeting TLR4 in normal human bronchial epithelial cells
样品类型: NHBE cells - 文献: Mediators of extracellular matrix degradation and inflammation: A new team of possible biomarkers for oral squamous cell carcinoma stage
样品类型: Oral cancer tissue - 文献: Mangiferin inhibits hypoxia/reoxygenation‑induced alveolar epithelial cell injury via the SIRT1/AMPK signaling pathway
样品类型: A549 cell - 文献: Comparison of safety, effectiveness and serum inflammatory factor indexes of Saccharomyces boulardii versus Bifidobacterium triple viable in treating children with chronic diarrhea: a randomized trial
样品类型: serum - 文献: LncRNA PMS2L2 downregulates miR-24 through methylation to suppress cell apoptosis in ulcerative colitis
样品类型: HCnEpCs - 文献: Knockdown of LINC01138 protects human chondrocytes against IL-1β-induced damage by regulating the hsa-miR-1207-5p/KIAA0101 axis
样品类型: Chondrocytes - 文献: Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India
样品类型: Serum - 文献: Serum profile of cytokines and their genetic variants in metabolic syndrome and healthy subjects: a comparative study
样品类型: serum - 文献: Exosomes derived from human umbilical cord mesenchymal stem cells alleviate acute liver failure by reducing the activity of the NLRP3 inflammasome in macrophages
样品类型: Serum - 文献: Interleukin-6 in Cerebrospinal Fluid Small Extracellular Vesicles as a Potential Biomarker for Prognosis of Aneurysmal Subarachnoid Haemorrhage
样品类型: Cerebrospinal Fluid - 文献: Adhesion molecules as diagnostic and severity biomarkers in pediatric community-acquired pneumonia
样品类型: Whole Blood,Serum - 文献: XIST silencing alleviated inflammation and mesangial cells proliferation in diabetic nephropathy by sponging miR-485
样品类型: Serum - 文献: Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles
样品类型: Cell culture supernatant - 文献: The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1 β Signaling Axis
样品类型: GES-1 cell - 文献: Upregulated NORAD is implicated in apoptosis, inflammation, and oxidative stress in ulcerative colitis through the nuclear factor-κappaB signaling
样品类型: human normal colonic mucosal cells (FHCs) - 文献: The effects of blood purification combined with antibiotics on extravascular lung water index, inflammatory factors, and prognosis of patients with severe acute pancreatitis complicated with acute respiratory distress syndrome.
样品类型: blood - 文献: Effect of probiotics supplementation combined with WeChat platform health management on nutritional status, inflammatory factors, and quality of life in patients with mild-to-moderate ulcerative colitis: a randomized trial.
样品类型: serum - 文献: CFNAs of RBCs affect the release of inflammatory factors through the expression of CaMKIV in macrophages
样品类型: macrophages - 文献: Predictors of coronary artery calcification and its association with cardiovascular events in patients with chronic kidney disease
样品类型: blood - 文献: Immune Cell and Biochemical Biomarkers in Advanced Laryngeal Cancer:
样品类型: serum - 文献: Correlation of Ionized Magnesium with the Parameters of Oxidative Stress as Potential Biomarkers in Patients with Anxiety and Depression: A Pilot Study:
样品类型: serum - 文献: The Anti-Inflammatory Effect of Smilax china L. Extract on LPS-Stimulated THP-1 via Downregulation of MAPK and NF-κB Signaling Pathway
样品类型: THP-1 cells - 文献: Downregulation of hsa_circ_0068087 ameliorates TLR4/NF-κB/NLRP3 inflammasome-mediated inflammation and endothelial cell dysfunction in high glucose conditioned by sponging miR-197
样品类型: Human Umbilical Vein Endothelial Cells (HUVECs) - 文献: Febuxostat Alleviates Allergic Rhinitis by Inhibiting Inflammation and Monocyte Adhesion in Human Nasal Epithelial Cells via Regulating KLF6.
样品类型: Human nasal epithelial cell (hNECs) - 文献: Effect of Thymosin on Inflammatory Factor Levels, Immune Function, and Quality of Life in Lung Cancer Patients Undergoing Radical Thoracoscopic Surgery
样品类型: Serum - 文献: PLD4 promotes M1 macrophages to perform antitumor effects in colon cancer cells
样品类型: macrophage - 文献: Circ_0101874 overexpression strengthens PDE4D expression by targeting miR-335-5p to promote neuronal injury in ischemic stroke
样品类型: SK-N-SH cells - 文献: Inhibition of microRNA-665 Alleviates Septic Acute Kidney Injury by Targeting Bcl-2
样品类型: HK-2 cell - 文献: Antidiabetic (type 2) effects of Lactobacillus G15 and Q14 in rats through regulation of intestinal permeability and microbiota
样品类型: Plasma,Serum - 文献: Long non-coding RNA MEG3 regulates CSE-induced apoptosis and inflammation via regulating miR-218 in 16HBE cells
样品类型: Cell culture supernatant - 文献: Plasma concentration of ZCCHC14 contributes to prognostic efficacy in intracerebral hemorrhage patients.
样品类型: Plasma - 文献: The effect of conventional versus electronic cigarette use on treatment outcomes of peri-implant disease
样品类型: Saliva - 文献: Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1–7) upregulation
样品类型: Cell culture supernatant,Serum - 文献: Role of the long non-coding RNA, SPRR2C, based on an in vitro psoriatic keratinocyte cell model
样品类型: Ker-CT cell,HaCaT cell - 文献: High anti-human cytomegalovirus antibody levels are associated with the progression of essential hypertension and target organ damage in Han Chinese population
样品类型: Serum - 文献: Depot-specific inflammation with decreased expression of ATM2 in white adipose tissues induced by high-margarine/lard intake
样品类型: Cell culture supernatant - 文献: Periodontal Ligament Stem Cells Reverse the High Glucose Level-Induced Inflammatory State of Macrophages
样品类型: THP-1 cell - 文献: Exploration of the mechanism by which Huangqi Guizhi Wuwu decoction inhibits Lps-induced inflammation by regulating macrophage polarization based on network pharmacology
样品类型: human histiocytic lymphoma (U-937) cell - 文献: Carotid intima-media thickness and serum proinflammatory cytokine levels in rosacea patients without cardiovascular risk factors
样品类型: Serum - 文献: Fluorometholone inhibits high glucose-induced cellular senescence in human retinal endothelial cells:
样品类型: human retinal endothelial cell - 文献: Differential expression of RNA-binding proteins in bronchial epithelium of stable COPD patients
样品类型: BEAS-2B cells - 文献: Association of PAR-2 Gene Polymorphisms with the Inflammatory Response and Susceptibility to Knee Osteoarthritis in the Chinese Han Population
样品类型: Synovia - 文献: Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells
样品类型: Cell culture supernatant - 文献: Blockade of 146b‑5p promotes inflammation in atherosclerosis‑associated foam cell formation by targeting TRAF6
样品类型: macrophages - 文献: Effect of laparoscopic sleeve gastrectomy on renal function in obese patients
样品类型: Serum,Urine - 文献: Frequency of interleukin-6 rs1800795 (-174G/C) and rs1800797 (-597G/A) polymorphisms in COVID-19 patients in Turkey who develop macrophage activation syndrome
样品类型: serum - 文献: MicroRNA-124 alleviates hyperoxia-induced inflammatory response in pulmonary epithelial cell by inhibiting TLR4/NF-κB/CCL2
样品类型: Beas2B cells - 文献: Risk factors for medication-related osteonecrosis of the jaw and salivary IL-6 IN cancer patients
样品类型: Saliva - 文献: Role of miR‑589‑3p in human lumbar disc degeneration and its potential mechanism
样品类型: Cell culture supernatant - 文献: N‑methyl pyrrolidone promotes ankle fracture healing by inhibiting inflammation via suppression of the mitogen‑activated protein kinase signaling pathway
样品类型: peripheral blood - 文献: Anti‑inflammatory effect of Resolvin D1 on LPS‑treated MG‑63 cells Corrigendum in /10.3892/etm.2019.8021
样品类型: Serum - 文献: Short‑term efficacy and safety of prednisone in herpes zoster and the effects on IL‑6 and IL‑10
样品类型: serum - 文献: Effects of propofol combined with remifentanil anesthesia on the NO, endothelin and inflammatory cytokines in the plasma of patients with liver cirrhosis during the perioperative period
样品类型: Plasma - 文献: Mitochondrial DNA-Induced Inflammatory Responses and Lung Injury in Thermal Injury Murine Model: Protective Effect of Cyclosporine-A
样品类型: Serum - 文献: Regulatory role and mechanism of the inhibition of the Mcl-1 pathway during apoptosis and polarization of H37Rv-infected macrophages
样品类型: Cell culture supernatant - 文献: Sex difference in IL-6 modulation of cognition among Chinese individuals with major depressive disorder
样品类型: Serum - 文献: A comparison of effects of scalp nerve block and local anesthetic infiltration on inflammatory response, hemodynamic response, and postoperative pain in patients undergoing craniotomy for cerebral aneurysms: a randomized controlled trial
样品类型: plasm - 文献: Tetrahydroxy stilbene glucoside ameliorates H2O2-induced human brain microvascular endothelial cell dysfunction in vitro by inhibiting oxidative stress and inflammatory responses
样品类型: Cell culture supernatant - 文献: miR-1287-5p upregulation inhibits the EMT and pro-inflammatory cytokines in LPS-induced human nasal epithelial cells (HNECs)
样品类型: HNECs cells - 文献: Maternal omega-3 polyunsaturated fatty acids supplementation in pregnancy decreases MMP-1 levels in breastmilk: a cross-sectional study
样品类型: Milk - 文献: Assessment of incidence of cerebral vascular diseases and prediction of stroke risk in chronic obstructive pulmonary disease patients using multimodal biomarkers
样品类型: serum - 文献: Systematically transplanted human gingiva‑derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis
样品类型: Serum - 文献: Curcumin affects ox‑LDL‑induced IL‑6, TNF‑α, MCP‑1 secretion and cholesterol efflux in THP‑1 cells by suppressing the TLR4/NF‑κB/miR33a signaling pathway
样品类型: Cell culture supernatant - 文献: Changes in expression of serum chemokine CXCL13 and IL-6 after hip replacement, and the relationship with lower limb vein thrombus
样品类型: Serum - 文献: Application of dexmedetomidine-assisted intravertebral anesthesia in hip replacement and its influence on T-lymphocyte subsets
样品类型: serum - 文献: Are Serum Interleukin 6 and Surfactant Protein D Levels Associated with the Clinical Course of COVID-19?
样品类型: Plasma - 文献: Chrysin protects human osteoarthritis chondrocytes by inhibiting inflammatory mediator expression via HMGB1 suppression
样品类型: Cell culture medium - 文献: A comparative study of autologous rectus fascia pubovaginal sling surgery and synthetic transobturator vaginal tape procedure in treatment of women with urodynamic stress urinary incontinence
样品类型: blood - 文献: TLR4/NF-κB signaling pathway gene single nucleotide polymorphisms alter gene expression levels and affect ARDS occurrence and prognosis outcomes
样品类型: plasma - 文献: A case-control study on the relationship between miRNAs single nucleotide polymorphisms and sepsis risk
样品类型: Serum - 文献: An unusual case of iron deficiency anemia is associated with extremely low level of transferrin receptor
样品类型: serum - 文献: Sinomenine Regulates Inflammatory Response and Oxidative Stress via Nuclear Factor kappa B (NF-κB) and NF-E2-Related Factor 2 (Nrf2) Signaling Pathways in Ankle Fractures in Children
样品类型: Serum - 文献: Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response
样品类型: Serum - 文献: Study of association of varicose veins and inflammation by inflammatory markers:
样品类型: Plasma - 文献: Avicularin Reduces the Expression of Mediators of Inflammation and Oxidative Stress in Bradykinin-Treated MG-63 Human Osteoblastic Osteosarcoma Cells
样品类型: Cell culture supernatant - 文献: Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1
样品类型: Cell culture supernatant - 文献: The effects of aerobic exercise and sertraline on pro-inflammatory indices and amelioration of neurotrophic factors in patients who have undergone CABG
样品类型: plasma,serum - 文献: The Association of Complements, TGF-β, and IL-6 with Disease Activity, Renal Damage, and Hematological Activity in Patients with Naïve SLE
样品类型: serum - 文献: Production of Inflammatory Mediators in Conditioned Medium of Adipose Tissue-Derived Mesenchymal Stem Cells (ATMSC)-Treated Fresh Frozen Plasma
样品类型: Adipose Tissue-Derived Mesenchymal Stem Cells (ATMSC) - 文献: Potential and Alternative Bioactive Compounds from Brown Agaricus bisporus Mushroom Extracts for Xerosis Treatment
样品类型: HaCaT cells - 文献: Long-Term Resistance–Endurance Combined Training Reduces Pro-Inflammatory Cytokines in Young Adult Females with Obesity
样品类型: serum - 文献: Evaluation of the relationship of serum vitamin D levels in COVID-19 patients with clinical course and prognosis.
样品类型: blood - 文献: Association between Interleukin-6 rs1800795 Polymorphism and Serum Interleukin-6 Levels and Full-Term Neonatal Sepsis
样品类型: serum - 文献: The role of zonulin in the pathogenesis of diabetic retinopathy
样品类型: serum - 文献: Oxidative stress index can be a new marker related to disease severity in COVID-19
样品类型: serum - 文献: Clinical efficacy and inflammatory factors in knee osteoarthritis with glucosamine hydrochloride plus meloxicam
样品类型: serum - 文献: TLR7 mediates increased vulnerability to ischemic acute kidney injury in diabetes
样品类型: Cell culture supernatant - 文献: Interleukin 8 as predictive factor for response to chemotherapy in colorectal cancer patients
样品类型: serum - 文献: Applied value of monitoring serum hepcidin in differential diagnosis of infection versus tumor fevers
样品类型: Plasma