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QuicKey Pro Rat CORT(Corticosterone) ELISA Kit

QuicKey Pro-大鼠皮质酮(CORT)酶联免疫吸附测定试剂盒

规格: / 96T / 48T / 96T*5
价格: / ¥2400 / ¥1650 / ¥10800

货号:E-OSEL-R0002

反应性: 大鼠

检测范围: 4.69~300 ng/mL

灵敏度: 1.87 ng/mL

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  • 文献(49)
  • QuicKey Pro系列

    与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。

    用途

    该试剂盒用于体外定量检测大鼠血清、血浆或其他相关生物液体中Cort浓度。

    检测原理

    本试剂盒采用竞争ELISA法。用大鼠 Cort抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗Cort单克隆抗体,样本(或标准品中)的Cort与包被抗原Cort竞争结合HRP标记的抗Cort单抗上的结合位点,形成包被抗原Cort-HRP标记的抗Cort单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Cort浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中Cort的浓度。

    反应类型 竞争法
    规格 96T
    反应时间 1.5h
    反应性 大鼠
    检测方法 Colormetric
    检测范围 4.69-300 ng/mL
    灵敏度 1.87 ng/mL
    样本体积 50μL
    样本类型 血清、血浆或其他相关生物液体

    特异性

    可检测样本中的大鼠Cort,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (ng/mL) O.D Average
    300 0.283
    0.333
    0.308
    150 0.447
    0.487
    0.467
    75 0.747
    0.719
    0.733
    37.5 1.108
    1.124
    1.116
    18.75 1.586
    1.57
    1.578
    9.380000000000001 1.941
    1.927
    1.934
    4.69 2.259
    2.265
    2.262
    0 2.659
    2.675
    2.667

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

      Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (ng/mL)
    13.64 37.16 121.75 14.17 37.85 113.64
    Standard
    deviation
    0.70 2.02 4.49 0.77 2.27 4.78
    C V (%) 5.12 5.43 3.69 5.41 6.00 4.21

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=8) 92-105 97
    EDTA plasma (n=8) 91-105 98

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

        Serum (n=5) EDTA plasma (n=5)
    1:2 Range (%) 94-106 96-111
    Average (%) 99 102
    1:4 Range (%) 89-102 91-103
    Average (%) 96 98
    1:8 Range (%) 93-107 98-111
    Average (%) 99 104
    1:16 Range (%) 95-111 96-112
    Average (%) 102 104

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存6个月;试剂盒组分开封后请按照下表中的条件分别保存。

    中文名称 英文名称 规格 保存条件
    ELISA 酶标板 Micro ELISA Plate (Dismountable) 96T:8 孔×12 条
    48T:8 孔×6 条
    24T:8 孔×3 条
    96T*5:5 块 96T酶标板
    2-8℃, 可存放1个月
    冻干标准品 Reference Standard 96T:2 支
    48T/24T:1 支
    96T*5:10 支
    2-8℃, 复溶后当天使用
    浓缩HRP酶结合抗体 (100×) Concentrated HRP Linked Detection Ab(100×) 96T:1支 60μL
    48T/24T:1支 30μL
    96T*5:5 支 60μL
    2-8℃(避光)
    标准品&样品稀释液 Reference Standard & Sample Diluent 96T/48T/24T:1瓶 10mL
    96T*5:5瓶 10mL
    2-8℃
    HRP酶结合抗体稀释液 HRP Linked Ab Diluent 96T/48T/24T:1瓶 7mL
    96T*5:5瓶 7mL
    酶结合物稀释液 Concentrated Wash Buffer(25×) 96T/48T/24T:1瓶 30mL
    96T*5:5瓶 30mL
    底物溶液(TMB) Substrate Reagent 96T/48T/24T:1瓶 10mL
    96T*5:5瓶 10mL
    2-8℃(避光)
    反应终止液 Stop Solution 96T/48T/24T:1瓶 10mL
    96T*5:5瓶 10mL
    2-8℃
    封板覆膜 Plate Sealer 96T/48T/24T:5张
    96T*5:25张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1.酶标仪(450nm波长滤光片)
    • 2.高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3.37℃恒温箱,双蒸水或去离子水
    • 4.吸水纸

    操作步骤

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