QuicKey Pro Rat CORT(Corticosterone) ELISA Kit
QuicKey Pro-大鼠皮质酮(CORT)酶联免疫吸附测定试剂盒
规格: | / 96T / 48T / 96T*5 |
价格: | / ¥2400 / ¥1650 / ¥10800 |
货号:E-OSEL-R0002
反应性: 大鼠
检测范围: 4.69~300 ng/mL
灵敏度: 1.87 ng/mL
QuicKey Pro系列
与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。
用途
该试剂盒用于体外定量检测大鼠血清、血浆或其他相关生物液体中Cort浓度。
检测原理
本试剂盒采用竞争ELISA法。用大鼠 Cort抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗Cort单克隆抗体,样本(或标准品中)的Cort与包被抗原Cort竞争结合HRP标记的抗Cort单抗上的结合位点,形成包被抗原Cort-HRP标记的抗Cort单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Cort浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中Cort的浓度。
反应类型 | 竞争法 |
规格 | 96T |
反应时间 | 1.5h |
反应性 | 大鼠 |
检测方法 | Colormetric |
检测范围 | 4.69-300 ng/mL |
灵敏度 | 1.87 ng/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆或其他相关生物液体 |
特异性
可检测样本中的大鼠Cort,且与其它相关蛋白无明显交叉反应。
重复性
板内,板间变异系数均<10%。
典型数据
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) |
13.64 | 37.16 | 121.75 | 14.17 | 37.85 | 113.64 |
Standard
deviation |
0.70 | 2.02 | 4.49 | 0.77 | 2.27 | 4.78 |
C V (%) | 5.12 | 5.43 | 3.69 | 5.41 | 6.00 | 4.21 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum (n=8) | 92-105 | 97 |
EDTA plasma (n=8) | 91-105 | 98 |
线性
分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。
Serum (n=5) | EDTA plasma (n=5) | ||
---|---|---|---|
1:2 | Range (%) | 94-106 | 96-111 |
Average (%) | 99 | 102 | |
1:4 | Range (%) | 89-102 | 91-103 |
Average (%) | 96 | 98 | |
1:8 | Range (%) | 93-107 | 98-111 |
Average (%) | 99 | 104 | |
1:16 | Range (%) | 95-111 | 96-112 |
Average (%) | 102 | 104 |
试剂盒组成及保存
未拆封的试剂盒可在2-8℃保存6个月;试剂盒组分开封后请按照下表中的条件分别保存。
中文名称 | 英文名称 | 规格 | 保存条件 |
---|---|---|---|
ELISA 酶标板 | Micro ELISA Plate (Dismountable) | 96T:8 孔×12 条 48T:8 孔×6 条 24T:8 孔×3 条 96T*5:5 块 96T酶标板 |
2-8℃, 可存放1个月 |
冻干标准品 | Reference Standard | 96T:2 支 48T/24T:1 支 96T*5:10 支 |
2-8℃, 复溶后当天使用 |
浓缩HRP酶结合抗体 (100×) | Concentrated HRP Linked Detection Ab(100×) | 96T:1支 60μL 48T/24T:1支 30μL 96T*5:5 支 60μL |
2-8℃(避光) |
标准品&样品稀释液 | Reference Standard & Sample Diluent | 96T/48T/24T:1瓶 10mL 96T*5:5瓶 10mL |
2-8℃ |
HRP酶结合抗体稀释液 | HRP Linked Ab Diluent | 96T/48T/24T:1瓶 7mL 96T*5:5瓶 7mL |
|
酶结合物稀释液 | Concentrated Wash Buffer(25×) | 96T/48T/24T:1瓶 30mL 96T*5:5瓶 30mL |
|
底物溶液(TMB) | Substrate Reagent | 96T/48T/24T:1瓶 10mL 96T*5:5瓶 10mL |
2-8℃(避光) |
反应终止液 | Stop Solution | 96T/48T/24T:1瓶 10mL 96T*5:5瓶 10mL |
2-8℃ |
封板覆膜 | Plate Sealer | 96T/48T/24T:5张 96T*5:25张 |
|
产品说明书 | Product Description | 1 份 | |
质检报告 | Certificate of Analysis | 1 份 |
说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。
试验所需自备物品
- 1.酶标仪(450nm波长滤光片)
- 2.高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3.37℃恒温箱,双蒸水或去离子水
- 4.吸水纸
操作步骤
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