Rat CASP3(Caspase 3) ELISA Kit
中文名称:大鼠胱天蛋白酶3(CASP3)酶联免疫吸附测定试剂盒
别称:
CASP3、CASP-3、CPP32、CPP32B、SCA-1、Apoptain、Yama
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 0.31-20 ng/mL
- 灵敏度: 0.19 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CASP3抗体包被于酶标板上,实验时样品(或标准品)中的大鼠CASP3会与包被抗体结合。后依次加入生物素化的抗大鼠CASP3抗体和辣根过氧化物酶标记的亲和素,抗大鼠CASP3抗体与结合在包被抗体上的大鼠CASP3结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,CASP3浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中CASP3的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 0.31-20 ng/mL |
灵敏度 | 0.19 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠CASP3,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Antioxidant and anti-apoptotic effects of tocotrienol-rich fraction against streptozotocin-induced diabetic retinopathy in rats
IF: 7.419 -
Glutamine prevents upregulation of NF-kB signaling and caspase 3 activation in ischaemia/reperfusion-induced testicular damage: An animal model
IF: 6.53 -
Escitalopram Targets Oxidative Stress, Caspase-3, BDNF and MeCP2 in the Hippocampus and Frontal Cortex of a Rat Model of Depression Induced by Chronic Unpredictable Mild Stress
IF: 5.924 -
Therapeutic Potentials of Selected Antihypertensive Agents and Their Fixed-Dose Combinations Against Trastuzumab-Mediated Cardiotoxicity
IF: 5.811Journal:Frontiers in Pharmacology -
Influence of furan and lead co-exposure at environmentally relevant concentrations on neurobehavioral performance, redox-regulatory system and apoptotic responses in rats
IF: 5.785Journal:ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY -
Selenium abates reproductive dysfunction via attenuation of biometal accumulation, oxido-inflammatory stress and caspase-3 activation in male rats exposed to arsenic
IF: 5.714 -
Atorvastatin-mediated downregulation of VCAM-1 and XO/UA/caspase 3 signaling averts oxidative damage and apoptosis induced by ovarian ischaemia/reperfusion injury
IF: 5.696 -
Banana peel ameliorated hepato-renal damage and exerted anti-inflammatory and anti-apoptotic effects in metal mixture mediated hepatic nephropathy by activation of Nrf2/ Hmox-1 and inhibition of Nfkb pathway
IF: 5.572 -
Lycopene Modulates Oxidative Stress and Inflammation in Hypercholesterolemic Rats
IF: 5.215Journal:Pharmaceuticals -
Plumbagin induces testicular damage via mitochondrial-dependent cell death
IF: 5.194