Rat IL-1β(Interleukin 1 Beta) ELISA Kit

产品货号:E-EL-R0012c

规格: / 96T / 48T
价格: / ¥2400 / ¥1650

货号:E-EL-R0012c

货期:3个工作日+顺丰周期

反应性: 大鼠

检测范围: 31.25~2000 pg/mL

灵敏度: 18.75 pg/mL

  • 详情
  • 文献(35)
  • 用途

    该试剂盒用于体外定量检测大鼠Serum, plasma and other biological fluids中天然及部分重组IL-1β浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上,实验时样品或标准品中的大鼠IL-1β会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素。抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-1β浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-1β的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性 大鼠
    检测方法 Colormetric
    检测范围 31.25—2000 pg/mL
    灵敏度 18.75 pg/mL
    样本体积 100μL
    样本类型 Serum, plasma and other biological fluids

    特异性

    可检测样本中的大鼠IL-1β,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    2000 2.388
    2.442
    2.415 2.349
    1000 1.602
    1.622
    1.612 1.546
    500 0.956
    0.942
    0.949 0.883
    250 0.494
    0.53
    0.512 0.446
    125 0.266
    0.242
    0.254 0.188
    62.5 0.169
    0.163
    0.166 0.1
    31.25 0.114
    0.122
    0.118 0.052
    0 0.06
    0.072
    0.066 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    100.50 314.20 880.10 107.40 340.60 954.80
    Standard
    deviation
    5.70 13.50 30.80 5.60 20.40 33.40
    C V (%) 5.67 4.30 3.50 5.21 5.99 3.50

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 85-98 91
    EDTA plasma (n=5) 86-100 93
    Cell culture media (n=5) 86-101 92

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 86-100 86-96 100-116
    Average (%) 92 91 106
    1:4 Range (%) 95-105 84-96 94-106
    Average (%) 100 91 100
    1:8 Range (%) 101-114 81-91 96-112
    Average (%) 108 86 103
    1:16 Range (%) 96-108 83-97 92-106
    Average (%) 102 89 99

    试剂盒组成及保存

    未拆封的试剂盒可在4℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 4℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 4℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 4℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1.对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟
    2.弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟
    3.弃掉板内液体,洗板3次
    4.每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次
    5.每孔加入90μL底物溶液,37℃孵育15分钟
    6.每孔加入50μL终止液
    7.立即在450nm波长下读数,处理数据

    客户发表文献

    1. 文献: Sun X S , Dong S Y , Li X Y , et al. Delivery of siRNA using folate receptor-targeted pH-sensitive polymeric nanoparticles for rheumatoid arthritis therapy[J]. Nanomedicine-Nanotechnology Biology and Medicine, 2019.
      样品类型: Plasma
    2. 文献: Lu H , Yao H , Zou R , et al. Galangin Suppresses Renal Inflammation via the Inhibition of NF-κB, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells[J]. BioMed Research International, 2019.
      样品类型: Cell culture supernatant
    3. 文献: Li M , Yu J , Zhao L , et al. Inhibition of macrophage migration inhibitory factor attenuates inflammation and fetal kidney injury in a rat model of acute pancreatitis in pregnancy[J]. International Immunopharmacology, 2019, 68: 106-144.
      样品类型: Serum
    4. 文献: Hong Y P , Deng W H , Guo W Y , et al. Inhibition of endoplasmic reticulum stress by 4-phenylbutyric acid prevents vital organ injury in rat acute pancreatitis[J]. Am J Physiol Gastrointest Liver Physiol, 2018, 315: G838-G847.
      样品类型: Serum
    5. 文献: Yu Z , Hongmiao X , Liang Z , et al. SB203580 attenuates acute lung injury and inflammation in rats with acute pancreatitis in pregnancy[J]. Inflammopharmacology, 2018.
      样品类型: Serum
    6. 文献: Nan Wang , et al. The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.Toxins, 2018, 10(11), 449.
      样品类型: Serum, Tissue homogenate
    7. 文献: Fan T, Yang S, Huang Z, et al. Autophagy Decreases Alveolar Epithelial Cell Injury by Suppressing the NF-κB Signaling Pathway and Regulating the Release of Inflammatory Mediators[J]. bioRxiv, 2018: 328039.
      样品类型: Tissue homogenate
    8. 文献: Nkpaa K W, Onyeso G I. Rutin Attenuates Neurobehavioral Deficits, Oxidative Stress, Neuro-Inflammation and Apoptosis in Fluoride Treated Rats[J]. Neuroscience Letters, 2018.
      样品类型: Tissue homogenate
    9. 文献: Zhao J, Zhang X, Sun X, et al. Dual-functional lipid polymeric hybrid pH-responsive nanoparticles decorated with cell penetrating peptide and folate for therapy against rheumatoid arthritis[J]. European Journal of Pharmaceutics and Biopharmaceutics, 2018, 130: 39-47.
      样品类型: Plasma
    10. 文献: Lu W, Wang L, Yao J, et al. C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization[J]. Molecular Medicine Reports, 2018, 18(2): 2110-2116.
      样品类型: Synovial fluid,Tissue homogenate
    11. 文献: Xia Y, Yu B, Ma C, et al. Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity[J]. Biomedicine & Pharmacotherapy, 2018, 106: 1332-1338.
      样品类型: Serum
    12. 文献: Meng L, Li L Y, Lu S, et al. The Protective Effect Of Dexmedetomidine On LPS-Induced Acute Lung Injury Through The HMGB1-Mediated TLR4/NF-κB And PI3K/Akt/mTOR Pathways[J]. Molecular Immunology, 2018, 94: 7-17.
      样品类型: Bronchoalveolar Lavage Fluid,Serum,Tissue homogenate
    13. 文献: Mi H, Dong Y, Zhang B, et al. Bifidobacterium Infantis Ameliorates Chemotherapy-Induced Intestinal Mucositis Via Regulating T Cell Immunity In Colorectal Cancer Rats[J]. Cellular Physiology and Biochemistry, 2017, 42(6): 2330-2341.
      样品类型: Serum
    14. 文献: Xie J C, Liu B L, Chen J L, et al. Umbilical Cord-Derived Mesenchymal Stem Cells Alleviated Inflammation And Inhibited Apoptosis In Interstitial Cystitis Via AKT/mTOR Signaling Pathway[J]. Biochemical And Biophysical Research Communications, 2017.
      样品类型: Tissue homogenate
    15. 文献: Osman W, Nurfarahin W, Lau S F, et al. Scopoletin-standardized Morinda Elliptica Leaf Extract Suppressed Inflammation And Cartilage Degradation To Alleviate Osteoarthritis: A preclinical Study[J]. Phytotherapy Research, 2017.
      样品类型: Serum
    16. 文献: Xun C, Mamat M, Guo H, et al. Tocotrienol Alleviates Inflammation And Oxidative Stress In A Rat Model Of Spinal Cord Injury Via Suppression Of Transforming Growth Factor-β[J]. Experimental and Therapeutic Medicine, 2017, 14(1): 431-438.
      样品类型: Serum
    17. 文献: Yeda X, Shaoqing L, Yayi H, et al. Dexmedetomidine Protects Against Renal Ischemia And Reperfusion Injury By Inhibiting The P38-MAPK/TXNIP Signaling Activation In Streptozotocin Induced Diabetic Rats[J]. Acta Cirurgica Brasileira, 2017, 32(6): 429-439.
      样品类型: Serum,Plasma
    18. 文献: Liu F, Chang X, Tian M, et al. Nano NIO Induced Liver Toxicity Via Activating The NF-κB Signaling Pathway In Rats[J]. Toxicology Research, 2017, 6(2): 242-250.
      样品类型: Tissue homogenate
    19. 文献: Borgohaina M P, Chowdhurya L, Ahmeda S, et al. Renoprotective And Antioxidative Effects Of Methanolic Paederia Foetida Leaf Extract On Experimental Diabetic Nephropathy In Rats[J]. Journal of Ethnopharmacology, 2017, 198: 451-459.
      样品类型: Serum,Tissue homogenate
    20. 文献: Liu H, Yang M, Pan L, et al. Hyperbaric Oxygen Intervention Modulates Early Brain Injury After Experimental Subarachnoid Hemorrhage In Rats: Possible Involvement Of TLR4/NF-κB-Mediated Signaling Pathway[J]. Cellular Physiology and Biochemistry, 2016, 38(6): 2323-2336.
      样品类型: Tissue homogenate
    21. 文献: Xiao Y D, Huang Y Y, Wang H X, et al. Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved In The Susceptibility To Ischemic Acute Kidney Injury In Diabetes[J]. Oxidative Medicine and Cellular Longevity, 2016.
      样品类型: Tissue homogenate
    22. 文献: Zhou Q, Pan X, Wang L, et al. The Protective Role Of Neuregulin-1: A Potential Therapy For Sepsis-Induced Cardiomyopathy[J]. European Journal of Pharmacology, 2016, 788: 234-240.
      样品类型: Serum
    23. 文献: Hang Z, Meng Z, Zhou F, et al. Cinnamaldehyde Ameliorates LPS-Induced Cardiac Dysfunction Via TLR4-NOX4 Pathway: The Regulation Of Autophagy And ROS Production[J]. Journal of Molecular and Cellular Cardiology, 2016, 101: 11-24.
      样品类型: Serum
    24. 文献: Fettucciari K, Ponsini P, Gioè D, et al. Enteric Glial Cells Are Susceptible To Clostridium Difficile Toxin B[J]. Cellular and Molecular Life Sciences, 2016.
      样品类型: Cell culture supernatant
    25. 文献: Borgohain M P, Lakhar M, Ahmed S, et al. Small Molecule Inhibiting NF-κB Ameliorates Oxidative Stress And Suppresses Renal Inflammation In Early Stage Of Alloxan-Induced Diabetic Nephropathy In Rat[J]. Basic & Clinical Pharmacology & Toxicology, 2016.
      样品类型: Tissue homogenate
    26. 文献: Bircan B, akr M, Krba S, et al. Effect Of Apelin Hormone On Renal Ischemia/Reperfusion Induced Oxidative Damage In Rats[J]. Renal Failure, 2016, 38(7): 1122-1128.
      样品类型: Serum
    27. 文献: Karamese M, Aydin H, Sengul E, et al. The Immunostimulatory Effect Of Lactic Acid Bacteria In A Rat Model[J]. Iranian Journal of Immunology, 2016, 13(3): 220-228.
      样品类型: Serum
    28. 文献: Bi Q R, Hou J J, Qi P, et al. Venenum Bufonis Induces Rat Neuroinflammation By Activiating NF-κB Pathway And Attenuation Of BDNF[J]. Journal of Ethnopharmacology, 2016, 186: 103-110.
      样品类型: Tissue homogenate
    29. 文献: Karamese M, Erol H S, Albayrak M, et al. Anti-Oxidant And Anti-Inflammatory Effects Of Apigenin In A Rat Model Of Sepsis: An Immunological, Biochemical, And Histopathological Study[J]. Immunopharmacology & Immunotoxicology, 2016, 38(3): 228-237.
      样品类型: Serum
    30. 文献: Yan Q, Li Y, Cheng N, et al. Effect Of Retinoic Acid On The Function Of Lipopolysaccharide-Stimulated Bone Marrow Stromal Cells Grown On Titanium Surfaces[J]. Inflammation Research, 2015, 64(1): 63-70.
      样品类型: Cell culture supernatant
    31. 文献: Luo Y, Fu C, Wang Z, et al. Asiaticoside Attenuates The Effects Of Spinal Cord Injury Through Antioxidant And AntiInflammatory Effects, And Inhibition Of The p38MAPK Mechanism[J]. Molecular Medicine Reports, 2015, 12: 8294-8300.
      样品类型: Serum
    32. 文献: Yang J, Liu X X, Fan H, et al. Extracellular Vesicles Derived From Bone Marrow Mesenchymal Stem Cells Protect Against Experimental Colitis Via Attenuating Colon Inflammation, Oxidative Stress And Apoptosis[J]. Plos One, 2015.
      样品类型: Tissue homogenate
    33. 文献: Deng Y, Kang W, Zhao J, et al. Osteoprotective Effect Of Echinocystic Acid, A Triterpone Component From Eclipta Prostrata, In Ovariectomy-Induced Osteoporotic Rats[J]. Plos One, 2015.
      样品类型: Serum
    34. 文献: Qin J, Ma X, Qi H, et al. Transplantation Of Induced Pluripotent Stem Cells Alleviates Cerebral Inflammation And Neural Damage In Hemorrhagic Stroke[J]. Plos One, 2015.
      样品类型: Tissue homogenate
    35. 文献: Zeng Z, Chen Z, Xu S, et al. Polydatin Alleviates Small Intestine Injury During Hemorrhagic Shock As A Sirt1 Activator[J]. Oxidative Medicine & Cellular Longevity, 2015.
      样品类型: Serum
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