Rat MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称:大鼠基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒
MMP9、CLG4B、Gelatinase B、GELB、MANDP2、92kDa Type IV Collagenase、92 KDa Gelatinase
Price:
- 反应性: Rat
- 检测范围: 7.81-500 ng/mL
- 灵敏度: 4.69 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠MMP-9抗体包被于酶标板上,实验时样品(或标准品)中的大鼠MMP-9会与包被抗体结合。后依次加入生物素化的抗大鼠MMP-9抗体和辣根过氧化物酶标记的亲和素,抗大鼠MMP-9抗体与结合在包被抗体上的大鼠MMP-9结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,MMP-9浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MMP-9的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 7.81-500 ng/mL |
灵敏度 | 4.69 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠MMP-9,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Fabrication of In Situ Layered Hydrogel Scaffold for the Co-delivery of PGDF-BB/Chlorhexidine to Regulate Proinflammatory Cytokines, Growth Factors, and MMP-9 in a Diabetic Skin Defect Albino Rat Model
IF: 6.988 -
Metabolites of gut microbiota fermenting Poria cocos polysaccharide alleviates chronic nonbacterial prostatitis in rats
IF: 6.953Journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULESPMID:35398386 -
Intragastric administration of Pien Tze Huang enhanced wound healing in diabetes by inhibiting inflammation and improving energy generation
IF: 6.656Journal:PHYTOMEDICINE -
The Activation of ROS/NF-κB/MMP-9 Pathway Promotes Calcium-Induced Kidney Crystal Deposition
IF: 6.543Journal:Oxidative Medicine and Cellular Longevity -
Huangbai liniment and berberine promoted wound healing in high-fat diet/Streptozotocin-induced diabetic rats
IF: 6.53 -
Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need
IF: 6.53 -
Water-soluble alkaloids extracted from Aconiti Radix lateralis praeparata protect against chronic heart failure in rats via a calcium signaling pathway
IF: 6.53 -
Protective Effect of Liposomal Epigallocatechin-Gallate in Experimental Gentamicin-Induced Hepatotoxicity
IF: 6.313 -
HGF and TSG-6 Released by Mesenchymal Stem Cells Attenuate Colon Radiation-Induced Fibrosis
IF: 5.924 -
The Mechanisms of Cucurbitacin E as a Neuroprotective and Memory-Enhancing Agent in a Cerebral Hypoperfusion Rat Model: Attenuation of Oxidative Stress, Inflammation, and Excitotoxicity.
IF: 5.811Journal:Frontiers in Pharmacology
Q1:Can ELISA measure the cell supernatant MMP-2 and MMP-9? I read the papers written by others, in addition to measuring the expression of these two indicators, but also to measure the activity, can ELISA measure the activity? What's the difference with gelatin enzymology?
These two kits can be used for the detection of your cell supernatant. However, ELISA detection of cell supernatants is affected by factors such as media components, cell growth, and external drug stimulation conditions, and it is recommended that you conduct a preliminary experiment before the formal experiment. Gelatin enzyme spectrometry uses the reversible combination of SDS and MMPs in the sample to separate MMP-2 and MMP-9 in the sample, and then restores the activity of both through the bivalent metal ion buffer system. This test method is specifically aimed at the activity detection of MMP-2 and MMP-9. ELISA detects the content (that is, concentration) of the tested substance through the binding reaction of antigen and antibody, that is, captures the MMP-2 and MMP-9 of the sample through the specific antibodies of MMP-2 and MMP-9. The experimental principles of the two are different, and the activity of MMP-2 and MMP-9 cannot be detected by ELISA.