Reactive Oxygen Species (ROS) Fluorometric Assay Kit
活性氧(ROS)荧光法测试盒
| 规格： | / 96T |
| 价格： | / ￥660 |
货号：E-BC-K138-F
检测仪器及波长: 荧光酶标仪（Ex/Em=500 nm/525 nm），流式细胞仪
样本类型: 组织细胞，培养细胞
检测原理
DCFH-DA（2,7-dichlorofuorescin diacetate）是一种可以自由穿过细胞膜的荧光探针，自身没有荧光，进入细胞内后，可以被细胞内的酯酶水解生成DCFH（dichlorofluorescin）。在活性氧存在时DCFH被氧化为不能透过细胞膜的强绿色荧光物质DCF（dichlorofluorescein），其荧光在激发波长502nm，发射波长530nm附近有最大波峰，强度与细胞内活性氧水平成正比。
关键信息
| 指标简称 | ROS, DCFH-DA, DCFH, DCF |
| 样本类型 | 组织细胞，培养细胞 |
| 检测仪器及波长 | 荧光酶标仪 |
| 保存条件 | Reagent1 -20℃，Reagent2 2-8℃ |
| 有效期 | 6个月 |
客户发表文献
- 文献: Compound Danshen Dripping Pill inhibits hypercholesterolemia/atherosclerosis-induced heart failure in ApoE and LDLR dual deficient mice via multiple mechanisms
样品类型: heart - 文献: Comparative toxicity reduction potential of UV/sodium percarbonate and UV/hydrogen peroxide treatments for bisphenol A in water: An integrated analysis using chemical, computational, biological, and metabolomic approaches
- 文献: Inhibition of ferroptosis attenuates oligospermia in male Nrf2 knockout mice
样品类型: seminal plasma - 文献: Alpinetin inhibits neuroinflammation and neuronal apoptosis via targeting the JAK2/STAT3 signaling pathway in spinal cord injury
样品类型: PC12 cell - 文献: Parental cadmium exposure during the spawning period reduces cadmium sensitivity through the antioxidant system in rare minnow (Gobiocypris rarus) larvae
样品类型: larvae - 文献: High-Fat-Diet–Induced Oxidative Stress Linked to the Increased Colonization of Lactobacillus sakei in an Obese Population | Microbiology Spectrum
样品类型: colon tissue - 文献: Mangosteen Pericarp Extract Supplementation Boosts Antioxidant Status via Rebuilding Gut Microbiota to Attenuate Motor Deficit in 6-OHDA-Induced Parkinson’s Disease
样品类型: Brain - 文献: Wnt inhibitory 1 ameliorates neovascularization and attenuates photoreceptor injury in an oxygen-induced retinopathy mouse model
样品类型: retinal cell - 文献: Glucose Deprivation Induced by Acarbose and Oncolytic Newcastle Disease Virus Promote Metabolic Oxidative Stress and Cell Death in a Breast Cancer Model
样品类型: mammary adenocarcinoma tissue - 文献: Nrf2-BDNF-TrkB pathway contributes to cortical hemorrhage-induced depression, but not sex differences:
样品类型: brain - 文献: Proteomic analyses revealed the antibacterial mechanism of Aronia melanocarpa isolated anthocyanins against Escherichia coli O157: H7
- 文献: The dispersion and utilization of lipid droplets mediates respiratory syncytial virus-induced airway hyperresponsiveness
样品类型: Beas-2b cell - 文献: Docetaxel prodrug and hematoporphyrin co-assembled nanoparticles for anti-tumor combination of chemotherapy and photodynamic therapy
- 文献: Potential Protective Mechanism in the Cardiac Microvascular Injury
样品类型: cardiac microvascular endothelial cells (CMECs) - 文献: CsCYT75B1, a Citrus CYTOCHROME P450 Gene, Is Involved in Accumulation of Antioxidant Flavonoids and Induces Drought Tolerance in Transgenic Arabidopsis
样品类型: leaf - 文献: Structural analysis and immunomodulatory activity of a homopolysaccharide isolated from Parabacteroides distasonis
样品类型: RAW 264.7 cell - 文献: Cytotoxic activity and influence on acetylcholinesterase of series dinuclear platinum(II) complexes with aromatic nitrogen-containing heterocyclic bridging ligands: Insights in the mechanisms of action
样品类型: MRC-5 cell,A375 cell,HeLa cell,PANC-1 cell - 文献: Verbenalin attenuates hepatic damage and mitochondrial dysfunction in alcohol-associated steatohepatitis by regulating MDMX/PPARα-mediated ferroptosis
样品类型: serum,Liver tissue,AML-12 cell - 文献: Discovery of novel tubulin inhibitors targeting the colchicine binding site via virtual screening, structural optimization and antitumor evaluation
- 文献: Metabolomic analysis reveals the responses of docosahexaenoic-acid-producing Schizochytrium under hyposalinity conditions
样品类型: microalgal cell - 文献: Inspired by bis-β-carboline alkaloids: Construction and antitumor evaluation of a novel bis-β-carboline scaffold as potent antitumor agents
样品类型: A549 cell - 文献: The rice bran peptide KF-8 extends the lifespan and improves the healthspan of Caenorhabditis elegans via skn-1 and daf-16
样品类型: L3 larvae - 文献: Vitamin C Improves the Outcomes of Cardiopulmonary Resuscitation and Alters Shedding of Syndecan‐1 and p38/MAPK Phosphorylation in a Rat Model
样品类型: Heart,brain - 文献: The mechanism underlying the TC-G 1008 rescue of reactive oxygen species (ROS)-induced osteoblast apoptosis by the upregulation of peroxiredoxin 1
样品类型: MC3T3-E1 cell - 文献: Discovery of potent tubulin inhibitors targeting the colchicine binding site via structure-based lead optimization and antitumor evaluation
样品类型: MDA-MB-231 cell - 文献: Patient-derived iPSCs link elevated mitochondrial respiratory complex I function to osteosarcoma in Rothmund-Thomson syndrome
样品类型: RTS fibroblast,FC fibroblast - 文献: Exendin-4 Attenuates Remodeling in the Remote Myocardium of Rats After an Acute Myocardial Infarction by Activating β-Arrestin-2, Protein Phosphatase 2A, and Glycogen Synthase Kinase-3 and Inhibiting β-Catenin
样品类型: Tissue homogenate - 文献: Glucose deprivation using 2-deoxyglucose and acarbose induce metabolic oxidative stress and apoptosis in female mice bearing breast cancer
样品类型: breast tumor tissue - 文献: Inhibition of ferroptosis attenuates busulfan-induced oligospermia in mice
样品类型: testicular tissue - 文献: Genotoxicity of chloroacetamide herbicides and their metabolites in vitro and in vivo
样品类型: Cell sample - 文献: Terminators or Guardians? Design, Synthesis, and Cytotoxicity Profiling of Chalcone-Sulfonamide Hybrids
样品类型: HCT116 cell - 文献: Didymin Ameliorates Liver Fibrosis by Alleviating Endoplasmic Reticulum Stress and Glycerophospholipid Metabolism: Based on Transcriptomics and Metabolomics
样品类型: liver - 文献: Ionizing Radiation Increases the Activity of Exosomal Secretory Pathway in MCF-7 Human Breast Cancer Cells: A Possible Way to Communicate Resistance against Radiotherapy
样品类型: Cells - 文献: Quercetin prevents myocardial infarction adverse remodeling in rats by attenuating TGF-β1/Smad3 signaling: Different mechanisms of action
样品类型: Tissue homogenate(heart) - 文献: The ameliorative effect of kaempferol against CdCl2- mediated renal damage entails activation of Nrf2 and inhibition of NF-kB
样品类型: kidney homogenates - 文献: Kaempferol prevents cadmium chloride-induced liver damage by upregulating Nrf2 and suppressing NF-κB and keap1
样品类型: liver - 文献: Inhibition of cardiac PERK signaling promotes peripartum cardiac dysfunction
样品类型: cardiomyocyte - 文献: Expression Profiling of Flavonoid Biosynthesis Genes and Secondary Metabolites Accumulation in Populus under Drought Stress
样品类型: leaf - 文献: Isovitexin protects against acute liver injury by targeting PTEN, PI3K and BiP via modification of m6A
样品类型: serum - 文献: Structural characterization and immunomodulatory activity of a glucan from Radix Codonopsis
样品类型: Cell culture supernatant - 文献: Indirect bilirubin impairs invasion of osteosarcoma cells via inhibiting the PI3K/AKT/MMP-2 signaling pathway by suppressing intracellular ROS
样品类型: 143B cell,MG63 cell - 文献: Cigarette Smoke Promotes Interleukin-8 Production in Alveolar Macrophages Through the Reactive Oxygen Species/Stromal Interaction Molecule 1/Ca2+ Axis
样品类型: Lung tissue - 文献: Neutrophil Extracellular Traps Induce Alveolar Macrophage Pyroptosis by Regulating NLRP3 Deubiquitination, Aggravating the Development of Septic Lung Injury
- 文献: Two resveratrol analogs, pinosylvin and 4,4′-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway
样品类型: Testicular - 文献: Melatonin alleviates angiotensin-II-induced cardiac hypertrophy via activating MICU1 pathway
样品类型: Primary cardiomyocytes (NMVMs) - 文献: Luteolin activates Tregs to promote IL-10 expression and alleviating caspase-11-dependent pyroptosis in sepsis-induced lung injury
样品类型: lung - 文献: Isoliquiritigenin alleviates P. gingivalis-LPS/ATP-induced pyroptosis by inhibiting NF-κB/ NLRP3/GSDMD signals in human gingival fibroblasts
- 文献: Daurisoline alleviated experimental colitis in vivo and in vitro: Involvement of NF-κB and Wnt/β-Catenin pathway
样品类型: RAW 264.7 cell - 文献: Testosterone promotion effect of Eucommia ulmoides staminate flower via the steroidogenic pathway and potential hormonal mechanism
- 文献: Chlorogenic acid alleviates neurobehavioral disorders and brain damage in focal ischemia animal models
样品类型: cerebral cortical - 文献: Chlorogenic acid alleviates cerebral ischemia-induced neuroinflammation via attenuating nuclear factor kappa B activation
样品类型: cerebral cortex tissue - 文献: Liraglutide inhibits receptor for advanced glycation end products (RAGE)/reduced form of nicotinamide-adenine dinucleotide phosphate (NAPDH) signaling to ameliorate non-alcoholic fatty liver disease (NAFLD) in vivo and vitro
样品类型: AML12 cell - 文献: Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5’AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells
样品类型: KLE cell - 文献: The mitochondria-targeted small molecule SS31 delays progression of behavioral deficits by attenuating β-amyloid plaque formation and mitochondrial/synaptic deterioration in APP/PS1 mice
样品类型: hippocampal - 文献: Effect of HIV-1 Protease Inhibitor on IL-18 and IL-1β in Rats with Insulinoma
样品类型: hippocampus tissue - 文献: 11S Glycinin Up-Regulated NLRP-3-Induced Pyroptosis by Triggering Reactive Oxygen Species in Porcine Intestinal Epithelial Cells
样品类型: IPEC-J2 cell - 文献: The anti-breast cancer property of physcion via oxidative stress-mediated mitochondrial apoptosis and immune response
样品类型: serum - 文献: Periostin regulates LPS-induced apoptosis via Nrf2/HO-1 pathway in periodontal ligament fibroblasts
样品类型: PDLFs - 文献: Specific Tumor Cell Detection by a Metabolically Targeted Aggregation-Induced Emission-Based Gold Nanoprobe
样品类型: MCF-7 cell,HepG2 cell,Caco-2 cell - 文献: Acupuncture Relieves Stress-Induced Depressive Behavior by Reducing Oxidative Stress and Neuroapoptosis in Rats
样品类型: hippocampus tissue - 文献: Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats
样品类型: hippocampal,hepatic - 文献: Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis
样品类型: Tissue homogenate - 文献: Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades
样品类型: cell - 文献: Epigallocatechin Gallate Alleviates Down-Regulation of Thioredoxin in Ischemic Brain Damage and Glutamate-Exposed Neuron
- 文献: Experimental Study of the Effect of Photobiomodulation Therapy on the Regulation of the Healing Process of Chronic Wounds
样品类型: serum - 文献: Ajania purpurea Extract Attenuates LPS-Induced Inflammation in RAW264.7 Cells and Peritonitis Mice
- 文献: Zhike pingchuan granules improve bronchial asthma by regulating the IL‑6/JAK2/STAT3 pathway
样品类型: lung - 文献: Pitavastatin maintains MAPK7 expression and alleviates angiotensin II‑induced vascular endothelial cell inflammation and injury
样品类型: HUVEC - 文献: Zanthoxylum bungeanum Seed Oil Attenuates LPS-Induced BEAS-2B Cell Activation and Inflammation by Inhibiting the TLR4/MyD88/NF-κB Signaling Pathway
样品类型: BEAS-2B cell - 文献: Main Active Components and Cell Cycle Regulation Mechanism of Astragali Radix and Angelicae Sinensis Radix in the Treatment of Ox-LDL-Induced HUVECs Injury and Inhibition of Their Cell Cycle
样品类型: HUVECs - 文献: Quercetin protects against experimentally-induced myocardial infarction in rats by an antioxidant potential and concomitant activation of signal transducer and activator of transcription 3
样品类型: left ventricles - 文献: Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains
样品类型: cortex - 文献: Protective effects of 1,2-benzisoxazole-3-methanesulfonamide (zonisamide)- loaded polymeric micelles against neurotoxicity in spinal cord: in vitro
样品类型: Cell lysate - 文献: Fisetin protects against streptozotocin-induced diabetic cardiomyopathy in rats by suppressing fatty acid oxidation and inhibiting protein kinase R
样品类型: Cell lysate - 文献: Normobaric hyperoxia re-sensitizes paclitaxel-resistant lung cancer cells
样品类型: A549 cell - 文献: Choline and citicoline ameliorate oxidative stress in acute kidney injury in rats.
样品类型: kidney tissue
