Recombinant Human AMPK1 protein (GST tag)
Price:
- 表达系统: E.coli
- 蛋白编码: Q13131-2
别称 |
PRKAA1;AMPK1;AMPK subunit alpha-1;PRKAA1;ACACA kinase;PRKAA1
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表达系统 |
E.coli
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序列 |
Lys 421-Gln 574
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蛋白编码 |
Q13131-2
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种属 |
Human
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计算分子量 |
41.8 kDa
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表观分子量 |
45 kDa
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标签 |
N-GST
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
内毒素 |
Please contact us for more information.
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
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背景 |
AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The catalytic alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinases LKB1 and CaMKK beta. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.
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实验操作视频
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