Recombinant Human GADD45γ/GADD45G Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： O95257
| 别称 |
Growth Arrest and DNA Damage-Inducible Protein GADD45 Gamma;Cytokine-Responsive Protein CR6;DNA Damage-Inducible Transcript 2 Protein;DDIT-2;GADD45G;CR6;DDIT2
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| 表达系统 |
E.coli
|
| 序列 |
Met 1-Glu159
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| 蛋白编码 |
O95257
|
| 种属 |
Human
|
| 计算分子量 |
19.3 kDa
|
| 表观分子量 |
21 kDa
|
| 标签 |
N-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Growth Arrest and DNA Damage-Inducible Protein GADD45 Υ (GADD45G) is a nuclear protein which belongs to the GADD45 family. GADD45G is highly expressed in placenta. GADD45G interacts with various proteins whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. GADD45G responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. GADD45G is also involved in the regulation of growth and apoptosis. GADD45G inhibits cell growth and differentiation by androgens. The mRNA expression is down-regulated in hepatocellular carcinoma.
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实验操作视频
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