Recombinant Human IL-8/CXCL8 Protein
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- 表达系统： E.coli
- 蛋白编码： P10145
- 应用性： Cell culture
| 别称 |
Interleukin-8;IL-8;C-X-C Motif Chemokine 8;Emoctakin;Granulocyte Chemotactic Protein 1;GCP-1;Monocyte-Derived Neutrophil Chemotactic Factor;MDNCF;Monocyte-Derived Neutrophil-Activating Peptide;MONAP;Neutrophil-Activating Protein 1;NAP-1;Protein 3-10C;T-Cell Chemotactic Factor;GCP1;IL8;Interleukin-8;LECT;LUCT;LYNAP;MDNCF;MONAP;NAF;NAP-1;NAP1
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| 表达系统 |
E.coli
|
| 序列 |
Ser28-Ser99
|
| 蛋白编码 |
P10145
|
| 种属 |
Human
|
| 计算分子量 |
9.3 kDa
|
| 表观分子量 |
11 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measure by its ability to chemoattract PBMC. The ED50 for this effect is <2 ng/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types; including T cells; endothelial cells and fibroblasts; in response to an inflammatory stimulus and acts by recruiting neutrophils; T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states.
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实验操作视频
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