Recombinant Human ITGA5 Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P08648
| 别称 |
Integrin Alpha-5;CD49 Antigen-Like Family Member E;Fibronectin Receptor Subunit Alpha;Integrin Alpha-F;VLA-5;CD49e;ITGA5;FNRA
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| 表达系统 |
HEK293 Cells
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| 序列 |
Phe42-Tyr995
|
| 蛋白编码 |
P08648
|
| 种属 |
Human
|
| 计算分子量 |
105.1 kDa
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| 表观分子量 |
130 kDa
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| 标签 |
C-His
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| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Integrin α-5 belongs to the Integrin α chain family and contains 7 FG-GAP repeats. Integrin α-5 joins with Integrin-β1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection; the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts; endothelial cells; peripheral T cells and platelets. Integrin α-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion; ITGA5 participates in cell-surface mediated signalling.
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实验操作视频
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