Recombinant Human/Mouse/Rat BMP-2 Protein
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- 表达系统： E.coli
- 蛋白编码： P12643
- 应用性： Cell culture
| 别称 |
Bone Morphogenetic Protein 2;BMP-2;Bone Morphogenetic Protein 2A;BMP-2A;BMP2;BMP2A
|
| 表达系统 |
E.coli
|
| 序列 |
Gln283-Arg396
|
| 蛋白编码 |
P12643
|
| 种属 |
Human/Mouse/Rat
|
| 计算分子量 |
13.8 kDa
|
| 表观分子量 |
14 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <1.0 μg/mL.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Bone Morphogenetic Protein-2 (BMP-2) is one of the bone-growth regulatory factors that belong to the
transforming growth factor-beta (TGF-beta) superfamily of proteins. BMPs are synthesized as large precursor
molecules, which are cleaved by proteolytic enzymes. The active form of BMP-2 can consist of a dimer of two
identical proteins or a heterodimer of two related bone morphogenetic proteins.
|
实验操作视频
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