Recombinant Human NCK1 Protein (His Tag)
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- 表达系统: E.coli
- 蛋白编码: P16333
别称 |
Cytoplasmic Protein NCK1;NCK adaptor Protein 1;Nck-1;SH2/SH3 Adaptor Protein NCK-Alpha;NCK1;NCK
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表达系统 |
E.coli
|
序列 |
Met 1-Ser377
|
蛋白编码 |
P16333
|
种属 |
Human
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计算分子量 |
45.0 kDa
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表观分子量 |
50 kDa
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标签 |
N-His
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纯度 |
> 80 % as determined by reducing SDS-PAGE.
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内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 5% Mannitol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
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背景 |
Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.
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实验操作视频
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